NEUROANATOMICAL ALTERATIONS IN THE RAT NUCLEUS OF THE SOLITARY TRACT FOLLOWING EARLY MATERNAL NACL DEPRIVATION AND SUBSEQUENT NACL REPLETION
NEUROANATOMICAL ALTERATIONS IN THE RAT NUCLEUS OF THE SOLITARY TRACT FOLLOWING EARLY MATERNAL NACL DEPRIVATION AND SUBSEQUENT NACL REPLETION
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DOI：
10.1002/cne.903330406
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发表时间：
1993-07-22
影响因子：
2.5
通讯作者：
HILL, DL
中科院分区：
文献类型：
--
作者：
KING, CT;HILL, DL
限制大鼠母体和断奶前饮食中的NaCl含量导致后代中鼓索神经对钠刺激的反应性显着和特异性降低（60%）。在出生后发育的任何时候补充膳食钠，都能使鼓索神经功能得到完全和持久的恢复。为了了解突触后细胞的成熟是否也受到早期饮食操作的影响，树突形态，对孤束核（NTS）内神经元和胶质细胞的密度和数量进行了研究，对Golgi-Cox染色的神经元树突形态的观察表明，NTS吻侧的多极梭形胞体细胞在发育过程中，在饮食剥夺NaCl后表现出较长的树突（剥夺大鼠）比对照组。这些变化一般维持在大鼠最初剥夺氯化钠，然后喂养NaCl-充满饮食断奶后（“恢复”大鼠）。相反，卵圆形神经元不受NaCl剥夺的影响，但在恢复后其树突的长度增加。沿着树突状细胞的改变，去NaCl大鼠的头侧孤束核神经元的堆积密度大于对照组，但恢复后与对照组相似。"神经胶质堆积密度在剥夺后也增加，并且在“恢复"大鼠中保持较高。这些结果表明，活动依赖性事件以及与传入活动无关的事件（例如，激素变化）可能影响NTS神经元的形态发育。此外，初级传入纤维，中枢神经元和胶质细胞之间的重要相互作用可能会直接在中央味觉系统的发展。
Restricting the NaCl content in the rat maternal and preweaning diet results in a significant and specific reduction (60%) of chorda tympani nerve responsiveness to sodium stimuli in the offspring. Repletion of dietary sodium at any time during postnatal development results in a complete and persistent recovery of chorda tympani nerve function. To learn whether the maturation of postsynaptic cells are also affected by the early dietary manipulation, dendritic morphology, neuronal and glial densities and numbers were studied within the area of the nucleus of the solitary tract (NTS).Examination of dendritic morphologies in Golgi-Cox stained neurons revealed that cells with multipolar and fusiform somata in the rostral NTS exhibited longer dendrites following dietary NaCl deprivation during development (deprived rats) than in controls. These changes were generally maintained in rats initially deprived of NaCl and then fed a NaCl-replete diet postweaning (''recovered'' rats). In contrast, ovoid neurons were not affected by NaCl deprivation but had increases in the lengths of their dendrites following ''recovery.'' Along with dendritic alterations, the packing density of neurons in the rostral NTS was greater in NaCl-deprived rats than in controls, but was similar to controls following ''recovery.'' Glial packing density also increased following deprivation and remained high in ''recovered'' rats.These results indicate that activity-dependent events as well as events not related to afferent activity (e.g., hormonal changes) may influence the morphological development of NTS neurons. In addition, significant interactions among primary afferent fibers, central neurons, and glia may direct development within the central gustatory system.