The compatibility of hepatocytes with chemically modified porous silicon with reference to in vitro biosensors
The compatibility of hepatocytes with chemically modified porous silicon with reference to in vitro biosensors
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DOI：
10.1016/j.biomaterials.2008.09.005
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发表时间：
2009-01-01
期刊：
影响因子：
14
通讯作者：
Sailor, Michael J.
中科院分区：
文献类型：
--
作者：
Alvarez, Sara D.;Derfus, Austin M.;Sailor, Michael J.
多孔硅是一种纳米结构材料，在分子和细胞生物传感、药物输送和组织工程应用中具有重要意义。表面化学是决定多孔硅在水介质中的稳定性、其对各种生物分子物种的亲和力以及与组织的相容性的重要因素。在这项研究中，一个主要的细胞类型的多孔硅样品含有各种表面化学的附着和生存能力的报告，和多孔硅膜的能力，以保持其光学反射性能相关的分子生物传感进行评估。研究了接枝到多孔Si表面的四种化学物质：氧化硅（通过臭氧氧化）、十二烷基（通过与十二碳烯的硅氢化反应）、十一烷酸（通过与十一碳烯酸的硅氢化反应）和低聚乙二醇（通过与十一碳烯酸的硅氢化反应，然后进行低聚乙二醇偶联反应）。傅里叶变换红外（FTIR）光谱和接触角测量用于表征表面。使用活体染料（钙黄绿素-AM和乙锭同型二聚体I）评估在这些表面上的原代大鼠肝细胞的粘附性和短期生存力，有和没有I型胶原蛋白的预吸附。在十一烷酸封端的多孔Si、氧化物封端的多孔Si和氧化物封端的平坦（无孔）Si上的细胞活力通过在8天的过程中定量白蛋白产生来监测。多孔硅薄膜在细胞培养8天后的稳定性进行了探讨，通过测量光学干涉反射光谱。结果表明，肝细胞粘附更好地与胶原蛋白包被的表面，和化学改性不会对原代大鼠肝细胞产生有害影响。氢化硅烷化化学大大提高了多孔Si与培养的原代细胞接触的稳定性，同时允许与组织培养聚苯乙烯上的标准培养制备物相当的细胞覆盖水平。(C)2008爱思唯尔有限公司保留所有权利。
Porous Si is a nanostructured material that is of interest for molecular and cell-based biosensing, drug delivery, and tissue engineering applications. Surface chemistry is an important factor determining the stability of porous Si in aqueous media, its affinity for various biomolecular species, and its compatibility with tissues. In this study, the attachment and viability of a primary cell type to porous Si samples containing various surface chemistries is reported, and the ability of the porous Si films to retain their optical reflectivity properties relevant to molecular biosensing is assessed. Four chemical species grafted to the porous Si surface are studied: silicon oxide (via ozone oxidation), dodecyl (via hydrosilylation with dodecene), undecanoic acid (via hydrosilylation with undecylenic acid), and oligo(ethylene) glycol (via hydrosilylation with undecylenic acid followed by an oligo(ethylene) glycol coupling reaction). Fourier Transform Infrared (FTIR) spectroscopy and contact angle measurements are used to characterize the surface. Adhesion and short-term viability of primary rat hepatocytes on these surfaces, with and without pre-adsorption of collagen type I, are assessed using vital dyes (calcein-AM and ethidium homodimer I). Cell viability on undecanoic acid-terminated porous Si, oxide-terminated porous Si, and oxide-terminated flat (non-porous) Si are monitored by quantification of albumin production over the course of 8 days. The stability of porous Si thin films after 8 days in cell culture is probed by measuring the optical interferometric reflectance spectra. Results show that hepatocytes adhere better to surfaces coated with collagen, and that chemical modification does not exert a deleterious effect on primary rat hepatocytes. The hydrosilylation chemistry greatly improves the stability of porous Si in contact with cultured primary cells while allowing cell coverage levels comparable to standard culture preparations on tissue culture polystyrene. (C) 2008 Elsevier Ltd. All rights reserved.