C-reactive protein enhances the respiratory burst of neutrophils-induced by antineutrophil cytoplasmic antibody.
C-reactive protein enhances the respiratory burst of neutrophils-induced by antineutrophil cytoplasmic antibody.
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DOI：
10.1016/j.molimm.2012.05.012
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发表时间：
2012-10
影响因子：
3.6
通讯作者：
Peng-Cheng Xu;J. Hao;Xiao-wei Yang;D. Chang;Min Chen;Ming-Hui Zhao
中科院分区：
文献类型：
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作者：
Peng-Cheng Xu;J. Hao;Xiao-wei Yang;D. Chang;Min Chen;Ming-Hui Zhao
血清C反应蛋白(CRP)是评价抗中性粒细胞胞浆抗体(ANCA)相关性小血管炎(AAV)病情活动性的有用生物标志物之一。越来越多的研究证明，CRP在多种疾病中都是致病的。在本研究中，我们用流式细胞术研究了CRP对中性粒细胞在ANCA诱导的呼吸爆发中的作用。如果反应系统中没有肿瘤坏死因子-α，ANCA只能诱导少量的中性粒细胞呼吸爆发。CRP可增强ANCA诱导的中性粒细胞呼吸爆发[平均荧光强度(MFI，68.45±16.87vs.58.65±15.09，P<；0.05)]或ANCA(MFI，79.51±15.90vs.61.73±14.89，P<；0.05)。C反应蛋白(50μg/mL，孵育30min)不能单独激活中性粒细胞，但与中性粒细胞孵育10min后，C反应蛋白(50μg/mL)可增加中性粒细胞膜蛋白3的表达(MFI值为365.27±143.50比235.32±124.65，P<0.05)。热处理的CRP不能提高ANCA诱导的中性粒细胞呼吸爆发的水平，也不能增加中性粒细胞膜蛋白3的表达。因此，CRP可激活中性粒细胞，增强ANCA诱导的呼吸爆发，可能是AAV的致病因素。
Serum C-reactive protein (CRP) was one of the useful biomarkers for evaluating the disease activity in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Cumulating studies proved that CRP was pathogenic in a variety of diseases. In the current study, the in vitro effects of CRP to prime neutrophils for ANCA-induced respiratory burst were investigated with flow cytometry. Without TNF-α in the reactive system, ANCA could only induce a slight level of respiratory burst of neutrophils. CRP could enhance the respiratory burst of neutrophils induced by ANCA against myeloperoxidse [mean fluorescence intensity (MFI, 68.45±16.87 vs. 58.65±15.09, P<0.05) or by ANCA against proteinase 3 (MFI, 79.51±15.90 vs. 61.73±14.89, P<0.05). Although CRP (50μg/mL, incubating for 30min) could not active neutrophils alone, after incubation with neutrophils for 10min, CRP (50μg/mL) could increase the expression of membrane proteinase 3 of neutrophils (MFI, 365.27±143.50 vs. 235.32±124.65, P<0.05). Heat-treated CRP could not enhance the levels of neutrophils respiratory burst induced by ANCA or increase the expression of membrane proteinase 3 of neutrophils. So CRP can prime neutrophils and enhance the respiratory burst induced by ANCA and might be pathogenic in AAV.