CD20, CD3, and CD40 Ligand Microclusters Segregate Three-Dimensionally In Vivo at B-Cell-T-Cell Immunological Synapses after Viral Immunity in Primate Brain
CD20, CD3, and CD40 Ligand Microclusters Segregate Three-Dimensionally In Vivo at B-Cell-T-Cell Immunological Synapses after Viral Immunity in Primate Brain
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DOI：
10.1128/jvi.01326-08
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发表时间：
2008-10-01
影响因子：
5.4
通讯作者：
Herrero, Maria-Trinidad
中科院分区：
文献类型：
--
作者：
Barcia, Carlos;Gomez, Aurora;Herrero, Maria-Trinidad
病毒感染细胞从大脑中的清除是由 T 细胞介导的，T 细胞与抗原呈递细胞结合，在免疫突触处形成超分子激活簇。然而，清除后，T 细胞仍保留在感染部位并保持局部激活。在目前的工作中，在病毒接种后 9 个月对猴子大脑中免疫细胞的长期相互作用进行了原位成像。病毒免疫后，在感染部位观察到T细胞和B细胞的持续浸润。 T 细胞因与 B 细胞接触而显示出 T 细胞受体信号传导的证据。 B 细胞-T 细胞突触的三维分析显示，T 细胞中存在 CD3 簇，B 细胞中存在 CD20 分离，涉及界面处 CD40 配体的募集。这些结果表明，B细胞和T细胞之间形成三维微簇的免疫突触发生在中枢神经系统体内，并表明这些相互作用可能参与原始感染部位病毒免疫后的淋巴细胞活化。
The clearance of virally infected cells from the brain is mediated by T cells that engage antigen-presenting cells to form supramolecular activation clusters at the immunological synapse. However, after clearance, the T cells persist at the infection site and remain activated locally. In the present work the long-term interactions of immune cells in brains of monkeys were imaged in situ 9 months after the viral inoculation. After viral immunity, the persistent infiltration of T cells and B cells was observed at the infection sites. T cells showed evidence of T-cell receptor signaling as a result of contacts with B cells. Three-dimensional analysis of B-cell-T-cell synapses showed clusters of CD3 in T cells and the segregation of CD20 in B cells, involving the recruitment of CD40 ligand at the interface. These results demonstrate that immunological synapses between B cells and T cells forming three-dimensional microclusters occur in vivo in the central nervous system and suggest that these interactions may be involved in the lymphocyte activation after viral immunity at the original infection site.