Succeed escape: Flow shear promotes tumbling of Escherichia colinear a solid surface.
Succeed escape: Flow shear promotes tumbling of Escherichia colinear a solid surface.
复制标题
成功逃脱：流动剪切促进大肠杆菌在固体表面共线翻滚。
DOI：
10.1038/srep35290
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发表时间：
2016
影响因子：
4.6
通讯作者：
Sheng,Jian
中科院分区：
文献类型：
--
作者：
Molaei,Mehdi;Sheng,Jian
了解细菌如何在各种刺激下移动接近表面对于广泛的微生物过程至关重要，包括生物膜形成，细菌运输和迁移。虽然之前的研究重点是单一刺激和细菌悬浮液之间的相互作用，但我们强调流动剪切和固体表面对细菌运动性的复合影响，特别是重新定向和翻滚。我们已经应用微流控和数字全息显微镜捕捉大量（>105）的3DEschericholitrajectories表面附近的各种流动剪切。我们发现，近表面的流动剪切促进细胞的重定向和减轻翻滚抑制和重定向限制发现在一个静止的流，从而提高表面正常的细菌分散。有条件的采样表明，两个免费的流体动力学机制，杰弗里轨道和剪切诱导的鞭毛解束，负责在细菌翻滚运动的增强。这些发现意味着流动剪切可以减轻细胞捕获并防止生物膜的形成。
Understanding how bacteria move close to a surface under various stimuli is crucial for a broad range of microbial processes including biofilm formation, bacterial transport and migration. While prior studies focus on interactions between single stimulus and bacterial suspension, we emphasize on compounding effects of flow shear and solid surfaces on bacterial motility, especially reorientation and tumble. We have applied microfluidics and digital holographic microscopy to capture a large number (>105) of 3DEscherichia colitrajectories near a surface under various flow shear. We find that near-surface flow shear promotes cell reorientation and mitigates the tumble suppression and re-orientation confinement found in a quiescent flow, and consequently enhances surface normal bacterial dispersion. Conditional sampling suggests that two complimentary hydrodynamic mechanisms, Jeffrey Orbit and shear-induced flagella unbundling, are responsible for the enhancement in bacterial tumble motility. These findings imply that flow shear may mitigate cell trapping and prevent biofilm initiation.