Determination of tetracycline antibiotics in animal tissues of food-producing animals by high-performance liquid chromatography using solid-phase extraction.
Determination of tetracycline antibiotics in animal tissues of food-producing animals by high-performance liquid chromatography using solid-phase extraction.
复制标题
采用固相萃取高效液相色谱法测定食用动物组织中的四环素抗生素。
DOI：
10.1016/0021-9673(94)80338-2
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Eva Matisová
中科院分区：
文献类型：
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作者：
Jozef Sokol;Eva Matisová
建立了高效液相色谱法测定牛和猪肌肉中土霉素（OTC）、四环素（TC）和金四环素（CTC）残留量的方法。该方法包括在EDTA-McIlvaine缓冲液中加入正己烷和二氯甲烷均质，离心，用三氯乙酸沉淀上清并过滤。在Spherisorb ODS 2柱（250 × 4 mm id）上，采用优化流动相0.01莫沙酸-乙腈-甲醇（45:35:20），在Separcol SI c18上进行预富集，提高了高效液相色谱分离的四环素类药物的清除率和回收率。采用360 nm紫外检测，检出限约为50 ng/g。二极管阵列光谱证实了该方法对四环素残留物研究的适用性。
A high-performance liquid chromatographic (HPLC) method was developed for the determination of oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) residues in bovine and porcine muscles. The method involved the homogenization of the sample in EDTA—McIlvaine buffer with addedn-hexane and dichloromethane, centrifugation, precipitation of the supernatant using trichloroacetic acid and filtration. Preconcentration on Separcol SI C18cartridges improved the clean-up and the recovery of tetracyclines that were separated by HPLC using the optimized mobile phase of 0.01Moxalic acid—acetonitrile—methanol (45:35:20) on a Spherisorb ODS 2 column (250 × 4 mm I.D.). UV detection at 360 nm was applied with a detection limit of about 50 ng/g. The diode-array spectra confirmed the applicability of this method to the study of tetracycline residues in carcasses.