Selective generation of leukotriene B4 by tracheal epithelial cells from dogs.
Selective generation of leukotriene B4 by tracheal epithelial cells from dogs.
复制标题
狗气管上皮细胞选择性产生白三烯 B4。
DOI：
10.1016/0006-291x(83)90671-x
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Goetzl,EJ
中科院分区：
文献类型：
--
作者：
Holtzman,MJ;Aizawa,H;Nadel,JA;Goetzl,EJ
将纯度大于95%的犬气管上皮细胞悬液与花生四烯酸(2 5~2 0 0μg/ml)孵育6 0~12 0min，最大白三烯B4 10 6细胞数为36 2±9 1皮摩尔，白三烯C4、D4和E4 10 6细胞数小于2 0皮摩尔，15、12和5-羟基二十碳四烯酸分别为10 30±4 6 3、76 7±5 0 0和32 4±10 0皮摩尔/10 6细胞(平均值±扫描电子显微镜，n=8)。白三烯B4的鉴定是通过层析和光谱性质、与单一特异性抗血浆的反应以及对中性粒细胞的趋化活性来确定的。因此，上皮细胞可能是肺气道炎性和过敏性介质的重要来源。
The incubation of suspensions of canine tracheal epithelial cells of greater than 95% purity with arachidonic acid (25–200 μg/ml) for 60–120 min resulted in the generation of a maximum of 36.2±9.1 picomoles of leukotriene B 4 10 6 cells, less than 2.0 picomoles of leukotrienes C 4, D 4, and E 4 10 6 cells, and 1030±463, 767±500, and 324±100 picomoles/10 6 cells of 15-, 12-, and 5-hydroxy-eicosatetraenoic acids, respectively (mean±SEM, n= 8). The identity of leukotriene B 4 was established by chromatographic and spectral properties, by reactivity with mono-specific anti-plasma, and by the chemotactic activity for neutrophils. Thus, the epithelium may be an important source of mediators of inflammation and hypersensitivity of pulmonary airways.