The effect of ACPD on the responses to NMDA and AMPA varies with layer in slices of rat visual cortex.
The effect of ACPD on the responses to NMDA and AMPA varies with layer in slices of rat visual cortex.
复制标题
ACPD 对 NMDA 和 AMPA 反应的影响随大鼠视觉皮层切片的层而变化。
DOI：
10.1016/s0006-8993(98)01000-2
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发表时间：
1998
期刊：
影响因子：
2.9
通讯作者：
Jin,X
中科院分区：
文献类型：
--
作者：
Wang,XF;Daw,NW;Jin,X
采用全细胞记录技术，观察了1S，3R-氨基环戊烷二元酸(ACPD)对大鼠视皮层各层细胞的作用。记录细胞的位置通过从pia到VI层/白质边界的距离来估计，并通过生物细胞素染色在34/97细胞中进行验证。代谢性谷氨酸激动剂acpd对NMDA和AMPA的反应的增强或抑制是通过靠近细胞体的药物的离子导入来检测的。ACPD的离子导入在不同的层次上有不同的效果。在第VI层，ACPD产生了显著的去极化，增强了对NMDA和AMPA的反应。在V层，Acpd不产生明显的去极化作用，但增强了对NMDA和AMPA的反应。在IV层，ACPD产生了一个小的超极化，并抑制了对NMDA的反应。在第二层和第三层，结果是小的和可变的。大多数染色细胞的记录来自锥体细胞。在获得非锥体细胞记录的情况下(3/34)，结果与来自同层锥体细胞的结果相同。当河豚毒素在浴液中时，也得到同样的结果。结论：ACPD对NMDA和AMPA反应的增强或抑制作用在大鼠视皮层不同层次上有所不同。
The effect of 1S,3R-aminocyclopentane dicarboxylic acid (ACPD) was measured on cells from various layers in slices of the rat visual cortex using whole-cell recording techniques. The position of the recorded cell was estimated by distance from pia to the layer VI/white matter boundary, and verified in 34/97 cells by staining with biocytin. Potentiation or depression of the responses to NMDA and AMPA by the metabotropic glutamate agonist ACPD was examined by iontophoresis of the drugs close to the cell body. Iontophoresis of ACPD had different effects in different layers. In layer VI, ACPD produced a substantial depolarization, which augmented the responses to NMDA and AMPA. In layer V, ACPD did not produce a significant depolarization, but potentiated the response to NMDA and AMPA. In layer IV, ACPD produced a small hyperpolarization, and depressed the response to NMDA. In layers II and III, the results were small and variable. Most recordings from stained cells were from pyramidal cells. Where recordings from non-pyramidal cells were obtained (3/34), results were the same as from pyramidal cells in the same layer. The same results were obtained when tetrodotoxin was in the bath solution. We conclude that the potentiation or depression of the response to NMDA and AMPA by ACPD varies with layer in rat visual cortex.