EARLY BLOOD-BRAIN-BARRIER DISRUPTION AFTER HIGH-DOSE SINGLE-FRACTION IRRADIATION IN RATS
EARLY BLOOD-BRAIN-BARRIER DISRUPTION AFTER HIGH-DOSE SINGLE-FRACTION IRRADIATION IN RATS
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DOI：
10.1007/bf01411440
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发表时间：
1995-01-01
影响因子：
2.4
通讯作者：
HAYAKAWA, T
中科院分区：
文献类型：
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作者：
NAKATA, H;YOSHIMINE, T;HAYAKAWA, T
研究了高剂量单组分辐照对大鼠脑血脑屏障通透性的影响。免疫组织化学与血清白蛋白抗体是检测内源性血清成分外渗的灵敏方法。20 Gy或40 Gy辐照后1天即发现白蛋白外渗。免疫反应性在第3天达到最大，随后几周逐渐下降，到第30天完全消失。80 Gy辐照后外渗明显增加，并在整个实验期间（6 d）持续增加。辐照后如此早的血脑屏障破坏以前没有文献记载。然而，观察到的血清白蛋白外渗的时间过程与先前的超微结构证据一致，即27 Gy照射后猴子脑血脑屏障通透性增加(4)。这种短暂的血脑屏障损伤可能导致放射术后可逆的神经系统症状。II也可能允许通常不能通过血脑屏障的药物通过血脑屏障，从而在此时给药时分散在大脑中。
We studied the effect of high-dose single-fraction irradiation on the permeability of the blood-brain barrier (BBB) in rat brains. Immunohistochemistry with an antibody to serum albumin was used as a sensitive method for detecting the extravasation of endogenous serum components. Extravasation of albumin was detected as early as 1 day after irradiation with 20 or 40 Gy. Immunoreactivity reached its maximum after 3 days, gradually decreased during the following few weeks and had disappeared by day 30. Extravasation was much greater after irradiation with 80 Gy and continued to increase during the whole period of the experiment (6 days). Disruption of BBB this early after irradiation has not been previously documented. The time course of observed serum albumin extravasation, however, agrees well with the previous ultrastructural evidence for increased BBB permeability after irradiation with 27 Gy in monkey brains(4). This transient impairment of BBB may contribute to the reversible neurological symptoms after radiosurgery. II may also allow drugs that normally not pass the BBB to do so and thus disperse in the brain when administered at this time.