Molecular and serological prevalence of Babesia bovis and Babesia bigemina in water buffaloes in the northeast region of Thailand
Molecular and serological prevalence of Babesia bovis and Babesia bigemina in water buffaloes in the northeast region of Thailand
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DOI：
10.1016/j.vetpar.2011.01.041
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发表时间：
2011-06-10
影响因子：
2.6
通讯作者：
Igarashi, Ikuo
中科院分区：
文献类型：
--
作者：
Terkawi, Mohamad Alaa;Nguyen Xuan Huyen;Igarashi, Ikuo
牛巴贝斯虫病是一种蜱传播的血液原虫病，主要由牛巴贝斯虫和双巴贝斯虫引起，在世界范围内具有很高的发病率和死亡率。该疾病在泰国东北部地区广泛传播，那里越来越多的牲畜由水牛组成。因此，本研究旨在调查泰国东北部水牛中牛双头牛双头牛的流行病学分布。采用巢式PCR （nPCR）、酶联免疫吸附试验（ELISA）和IFAT技术，在5个省份随机采集305份水牛血样，同时进行分析。nPCR结果显示，牛b型和双胎b型的总感染率分别为11.2%和3.6%，ELISA结果为14.7%和5.9%，IFAT结果为16.8%和5.6%。分子检测结果与血清学检测结果高度一致，表明该诊断方法的特异性和敏感性以及寄生虫在调查地区的地方性稳定性。两种感染的流行在年龄和地点而非性别的基础上观察到统计学上的显著差异。我们的数据为泰国东北部地区水牛b型牛和b型双头牛感染的流行病学提供了有价值的信息，这可能对该疾病的管理和控制计划非常有益。(C) 2011 Elsevier B.V.版权所有
Bovine babesiosis is a tick-transmitted hemoprotozoan disease that is mainly caused by Babesia bovis and Babesia bigemina and is characterized by significant morbidity and mortality worldwide. The disease is widespread in the northeastern region of Thailand, where an increasingly large part of the livestock is composed of water buffaloes. The present study was therefore conducted to investigate the epidemiological distribution of B. bovis and B. bigemina in water buffaloes in the northeastern region of Thailand. A total of 305 buffalo blood samples were randomly collected from five provinces and simultaneously analyzed by the nested PCR (nPCR) assay, ELISA, and IFAT techniques. The overall prevalence of B. bovis and B. bigemina was 11.2% and 3.6% by nPCR, 14.7% and 5.9% by ELISA, and 16.8% and 5.6% by IFAT, respectively. The high concordance between the molecular and the serological detection tests revealed the specificity and sensitivity of the diagnostic assays used for the detection of infection as well as the endemic stability status of the parasites in the surveyed areas. Statistically significant differences in the prevalence of the two infections were observed on the basis of age and location but not gender. Our data provide valuable information regarding the epidemiology of B. bovis and B. bigemina infection in water buffaloes in the northeastern region of Thailand which will likely be very beneficial for management and control programs of this disease. (C) 2011 Elsevier B.V. All rights reserved.