Egg Yolk Antibodies (IgY) in Routine Diagnostic Work.

Egg Yolk Antibodies (IgY) in Routine Diagnostic Work.
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发表时间:
1996
期刊:
ALTEX
影响因子:
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通讯作者:
C. Staak
C. Staak
中科院分区:
其他
文献类型:
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作者:
C. Staak

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根据Linscott's Directory of Immunological and Biological Reagents(1994/95),卵黄抗体(IgY)的商业可用性极低。对于IgY的制备、清洁和检测,拥有类似于哺乳动物抗体的蛋白A和蛋白G的“蛋白Y”将是有利的。到目前为止,对“蛋白质Y”的搜索是不成功的。IgY已用于常规诊断工作,包括以下主题:1.从吸血昆虫的腹部血液中鉴定宿主物种; 2.用于对杜伦进行ELISA的IgY-抗马-Ig-PO缀合物; 3.用于狂犬病诊断工作的FITC-缀合的IgY-抗狂犬病抗体; 4.抗禽病毒病的FITC-缀合的IgY(纽卡斯尔dis.,传染性支气管炎,Gumboro)。在所有情况下都取得了令人满意的结果。
According to Linscott"s Directory of Immunological and Biological Reagents (1994/95) the commercial availability of egg-yolk antibodies (IgY) is extremely low. For preparation, cleaning and detection of IgY it would be of advantage to have a "Protein Y" available analogous to protein A and protein G for mammalian antibodies. Until now, the search for "Protein Y" was unsuccessful. IgY has been used for routine diagnostic work covering the following subjects: 1.Identification of the host species from abdominal blood of haematophageous insects; 2.IgY-anti-horse-Ig-PO conjugate for ELISA on dourine; 3.FITC-conjugated IgY-antirabies for diagnostic work on rabies; 4.FITC-conjugated IgY against avian virus diseases (Newcastle dis., Infectious bronchitis, Gumboro). In all cases satisfactory results have been achieved.