The basic helix-loop-helix protein family: Comparative genomics and phylogenetic analysis
The basic helix-loop-helix protein family: Comparative genomics and phylogenetic analysis
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DOI：
10.1101/gr.177001
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发表时间：
2001-05-01
期刊：
影响因子：
7
通讯作者：
Vervoort, M
中科院分区：
文献类型：
--
作者：
Ledent, V;Vervoort, M
碱性螺旋环抑制蛋白（bHLH）是一类转录因子，在包括果蝇、线虫和脊椎动物在内的多种后生动物的发育过程中发挥重要作用。它们也参与人类疾病，特别是癌症发生。我们在秀丽隐杆线虫和黑腹果蝇的完全测序的基因组中广泛搜索bHLH序列。我们分别发现了35和56个不同的基因，它们可能代表了这些生物体的完整bHLH基因组。这些基因的系统发育分析，以及大量的（>350）从其他来源的bHLH，导致trs定义44个直系亲属的家庭，其中36个包括bHLH只从动物，和两个有代表性的酵母和动物。此外，我们确定了两个bHLH基序只存在于酵母中，和四个聊天只存在于植物中;然而，后者的数字肯定是低估了。大多数动物家族（35/38）包含苍蝇、线虫和脊椎动物基因，这表明它们生活在前寒武纪时代（6亿年前）的共同祖先已经拥有多达35种不同的bHLH基因。
The basic Helix-Loop-Helix (bHLH) proteins are transcription factors that play important roles during the development of various metazoans including fly, nematode, and vertebrates. They are also involved in human diseases, particularly in cancerogenesis. We made an extensive search for bHLH sequences in the completely sequenced genomes of Caenorhabditis elegans and of Drosophila melanogaster. We found 35 and 56 different genes, respectively, which may represent the complete set of bHLH of these organisms. A phylogenetic analysis of these genes, together with a large number (>350) of bHLH from other sources, led trs to define 44 orthologous families among which 36 include bHLH from animals only, and two have representatives in both yeasts and animals. In addition, we identified two bHLH motifs present only in yeast, and four chat are present only in plants; however, the latter number is certainly an underestimate. Most animal families (35/38) comprise fly, nematode, and vertebrate genes, suggesting that their common ancestor, which lived in pre-Cambrian times (600 million years ago) already owned as many as 35 different bHLH genes.