Provision of a voluntary exercise environment enhances running activity and prevents obesity in Snark-deficient mice
Provision of a voluntary exercise environment enhances running activity and prevents obesity in Snark-deficient mice
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DOI：
10.1152/ajpendo.90891.2008
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发表时间：
2009-05-01
影响因子：
5.1
通讯作者：
Esumi, Hiroyasu
中科院分区：
文献类型：
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作者：
Ichinoseki-Sekine, Noriko;Naito, Hisashi;Esumi, Hiroyasu
Ichinoseki-Sekine N，Naito H，Tsuchihara K，小林I，Ogura Y，Kakigi R，KurochiM，Fujioka R，Esumi H.提供一个自愿的运动环境，增强了Snark缺陷小鼠的跑步活动，并防止肥胖。Am J Physiol Endocrinol Metab 296：E1013-E1021，2009。首次发表于2009年3月10日; doi：10.1152/ajpendo.90891.2008。本研究旨在研究SNARK在小鼠体力活动水平中的参与。为了检查SNARK缺陷对自主跑步的急性影响，将Snark缺陷小鼠（Snark（+/-）：n = 16）和它们的野生型对应物（Snark（+/-）：n = 16）分配到久坐或运动（1周自主轮跑）组。此外，为了阐明基因型之间自主跑步活动及其效果的差异，还将小鼠（Snark（+/-）：n = 16; Snark（+/-）：n = 16）在具有/不具有跑步轮的单独笼子中保持5个月。出乎意料的是，在两个自愿跑步实验中，与Snark（+/-）小鼠相比，Snark（+/-）小鼠的跑步距离增加。在久坐条件下，Snark（+/-）小鼠的体重和白色脂肪组织重量显著增加。但在长期实验中，运动条件下两种基因型之间没有观察到显著差异，并且值显著小于久坐条件下的值。短期实验中，运动组与安静组血清IL-6水平无显著差异，而安静组血清IL-6水平显著低于其他各组。相反，运动Snark（+/-）小鼠的血清瘦素水平显著低于久坐Snark（+/-）小鼠。这项研究的结果表明，暴露在允许自愿运动的环境中可以促进Snark缺陷小鼠的跑步活动增加并防止肥胖。
Ichinoseki-Sekine N, Naito H, Tsuchihara K, Kobayashi I, Ogura Y, Kakigi R, Kurosaka M, Fujioka R, Esumi H. Provision of a voluntary exercise environment enhances running activity and prevents obesity in Snark-deficient mice. Am J Physiol Endocrinol Metab 296: E1013-E1021, 2009. First published March 10, 2009; doi:10.1152/ajpendo.90891.2008.-The present study was performed to investigate the involvement of SNARK in physical activity levels in mice. To examine the acute effect of SNARK deficiency on voluntary running, Snark-deficient mice (Snark(+/-): n = 16) and their wild-type counterparts (Snark(+/-): n = 16) were assigned to sedentary or exercise (1 wk voluntary wheel running) groups. In addition, to clarify the differences in voluntary running activity and its effect between genotypes, mice (Snark(+/-): n = 16; Snark(+/-): n = 16) were also kept in individual cages with/without a running wheel for 5 mo. Unexpectedly, in both voluntary running experiments, running distances were increased in Snark(+/-) mice compared with Snark(+/-) mice. Under sedentary conditions, body and white adipose tissue weights were increased significantly in Snark(+/-) mice. However, no significant differences were observed between the two genotypes under exercise conditions, and the values were significantly less than those under sedentary conditions in the long-term experiment. In the short-term experiment, serum interleukin-6 level in exercised Snark(+/+) mice was the same as that in sedentary Snark(+/+) mice, whereas that in sedentary Snark(+/-) mice was significantly lower than in the other groups. In contrast, serum leptin level was reduced significantly in exercised Snark(+/-) mice compared with sedentary Snark(+/-) mice. The results of this study demonstrated that exposure to an environment that allows voluntary exercise promotes increased running activity and prevents obesity in Snark-deficient mice.