Increased frequency of antral CD4+ T and CD19+ B cells in patients with Helicobacter pylori-related peptic ulcer disease
Increased frequency of antral CD4+ T and CD19+ B cells in patients with Helicobacter pylori-related peptic ulcer disease
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DOI：
10.1111/j.0300-9475.2005.01537.x
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发表时间：
2005-01-01
影响因子：
3.7
通讯作者：
Florholmen, J
中科院分区：
文献类型：
--
作者：
Goll, R;Husebekk, A;Florholmen, J
只有一小部分幽门螺杆菌（HP）感染的个体发展为临床疾病。最近的研究表明，免疫机制可能是重要的了解HP感染的病理生理。个体细胞免疫应答的差异可能反映了临床多样性。本研究的目的是调查在三个临床明确定义的患者组：HP阳性消化性溃疡，HP阳性和HP阴性胃炎的细胞免疫反应。对胃粘膜活检进行流式细胞术和组织学分析。HP阳性的消化性溃疡患者T淋巴细胞（CD 3（+））数（13.8%）明显高于HP阳性的非溃疡性胃炎患者（6.3%）。还观察到B淋巴细胞（CD 19（+））无显著性增加。HP溃疡组和非溃疡性HP胃炎组粘膜CD_4/CD_8比值分别为2.4和1.0，差异有统计学意义。因此，消化性溃疡患者胃粘膜中以B细胞（CD 19（+））和辅助性T细胞（CD 4（+））为主，非溃疡患者胃粘膜中以细胞毒性T细胞（CD 8（+））为主。总之，粘膜淋巴细胞的T细胞亚群分布的显着差异，检测HP感染的患者，强烈相关的存在或不存在消化性溃疡。
Only a fraction of Helicobacter pylori (HP)-infected individuals develop clinical disease. Recent research indicates that immunological mechanisms may be important for understanding the pathophysiology of HP infection. Differences in the individual cellular immune response may reflect the clinical diversity. The aim of the present study was to investigate the cellular immune response against HP in three clinically well-defined patient groups: HP-positive peptic ulcer, HP-positive and HP-negative gastritis. Biopsies from gastric mucosa were processed for analysis by flow cytometry and histology. The number of T lymphocytes (CD3(+)) was significantly higher in HP-positive peptic ulcer (13.8%) than in HP-positive nonulcer gastritis (6.3%). A nonsignificant increase for B lymphocytes (CD19(+)) was noted as well. Furthermore, a significant difference was seen in mucosal CD4/CD8 ratio between HP ulcer (2.4) and nonulcer HP gastritis (1.0) patients. Thus, B cells (CD19(+)) and T-helper cells (CD4(+)) were dominant in gastric mucosa from peptic ulcer patients, and cytotoxic T cells (CD8(+)) were relatively dominant in gastric mucosa from nonulcer patients. In conclusion, distinct differences in the T-cell subset distribution of mucosal lymphocytes were detected in patients with HP infection, strongly correlated with the presence or absence of peptic ulcer.