Determination of the Absolute Configuration of Peptide Natural Products by Using Stable Isotope Labeling and Mass Spectrometry
Determination of the Absolute Configuration of Peptide Natural Products by Using Stable Isotope Labeling and Mass Spectrometry
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DOI：
10.1002/chem.201103479
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发表时间：
2012-02-01
影响因子：
4.3
通讯作者：
Gruen, Peter
中科院分区：
文献类型：
--
作者：
Bode, Helge B.;Reimer, Daniela;Gruen, Peter
包括绝对构型在内的天然产物的结构鉴定是一项复杂的任务，涉及不同的分析方法，如质谱学、核磁共振谱和化学衍生化，这些方法通常在分离目标化合物后进行。在这里，结合稳定的同位素标记和Xnowhabdus菌株及其转氨酶突变体和详细的MS分析，可以在不实际分离的情况下阐明名为GameX的新型环肽的结构，包括它们在粗提物中的绝对构型。
Structure elucidation of natural products including the absolute configuration is a complex task that involves different analytical methods like mass spectrometry, NMR spectroscopy, and chemical derivation, which are usually performed after the isolation of the compound of interest. Here, a combination of stable isotope labeling of Photorhabdus and Xenorhabdus strains and their transaminase mutants followed by detailed MS analysis enabled the structure elucidation of novel cyclopeptides named GameXPeptides including their absolute configuration in crude extracts without their actual isolation.