A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas.
A new gene coding for a differentiation antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas.
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DOI：
10.1084/jem.180.1.35
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发表时间：
1994-07-01
期刊：
影响因子：
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通讯作者：
Boon T
中科院分区：
文献类型：
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作者：
Coulie PG;Brichard V;Van Pel A;Wölfel T;Schneider J;Traversari C;Mattei S;De Plaen E;Lurquin C;Szikora JP;Renauld JC;Boon T
有报道称，抗肿瘤细胞溶解T淋巴细胞(CTL)克隆可以从人类白细胞抗原A2黑色素瘤患者的外周血淋巴细胞中分离出来，在体外经自体肿瘤细胞刺激后，其中一些细胞毒性T淋巴细胞克隆可溶解大部分的人类白细胞抗原A2黑色素瘤。这种CTL克隆识别的第一个抗原先前被证明是由酪氨酸酶基因编码的。我们在这里报告了另一个基因的鉴定，该基因也指导了大多数黑色素瘤上被人类白细胞抗原A2所限制的CTL克隆所识别的抗原的表达。该基因被命名为Melan-A，与任何已知基因无关。该基因全长18kb，由5个外显子组成。像酪氨酸酶基因一样，它在大多数黑色素瘤肿瘤样本中都有表达，而在正常细胞中，只有黑素细胞中有表达。
It has been reported previously that antitumor cytolytic T lymphocyte (CTL) clones can be isolated from blood lymphocytes of HLA-A2 melanoma patients, after stimulation in vitro with autologous tumor cells, and that some of these CTL clones lyse most HLA-A2 melanomas. A first antigen recognized by such CTL clones was previously shown to be encoded by the tyrosinase gene. We report here the identification of another gene that also directs the expression of an antigen recognized on most melanomas by CTL clones that are restricted by HLA-A2. The gene, designated Melan-A, is unrelated to any known gene. It is 18 kb long and comprises five exons. Like the tyrosinase gene, it is expressed in most melanoma tumor samples and, among normal cells, only in melanocytes.