Expression of RANTES mRNA and protein in airways of patients with mild asthma
Expression of RANTES mRNA and protein in airways of patients with mild asthma
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DOI：
10.1164/ajrccm.154.6.8970374
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发表时间：
1996-12-01
影响因子：
24.7
通讯作者：
Chung, KF
中科院分区：
文献类型：
--
作者：
Berkman, N;Krishnan, VL;Chung, KF
趋化细胞因子（趋化因子）如RANTES是嗜酸性粒细胞、T淋巴细胞和单核细胞/巨噬细胞的有效趋化剂。我们检测了仅接受β 2-肾上腺素能激动剂治疗的轻度哮喘患者支气管活检和支气管肺泡灌洗（BAL）细胞中RANTES mRNA和蛋白的表达。使用定量聚合酶链反应（PCR），哮喘患者的平均RANTES起始cDNA为110 +/- 18 fg/pg β-肌动蛋白，而正常人为33.7 +/- 11.0 fg/pg（p < 0.003），但两组BAL细胞中RANTES mRNA表达无显著差异。支气管活检组织的冷冻切片的免疫组织化学染色与抗RANTES抗体使用过氧化物酶抗过氧化物酶的方法显示气道上皮细胞，气道平滑肌和上皮下细胞的组成性染色。然而，尽管哮喘患者粘膜活检组织中CD 4（+）T细胞、CD 68（+）巨噬细胞和MBP（+）嗜酸性粒细胞的数量显著增加，但正常受试者和哮喘患者之间RANTES染色细胞的数量没有差异。双重染色显示RANTES在CD 3（+）T淋巴细胞中的表达比例。因此，RANTES在气道中组成性表达，并且RANTES mRNA在轻度哮喘患者的气道中升高，支持RANTES在正常和哮喘气道中的作用。
Chemoattractant cytokines (chemokines) such as RANTES, are potent chemoattractants for eosinophils, T lymphocytes, and monocyte/macrophages. We examined RANTES mRNA and protein expression in bronchial biopsies and bronchoalveolar lavage (BAL) cells from patients with mild asthma on beta(2)-adrenergic agonist therapy only. Using quantitative polymerase chain reaction (PCR), mean RANTES starting cDNA was 110 +/- 18 fg/pg beta-actin in asthmatics compared with 33.7 +/- 11.0 fg/pg in normals (p < 0.003) but there was no significant difference in RANTES mRNA expression in BAL cells between the two groups. Immunohistochemical staining of cryostat sections of bronchial biopsies with an anti-RANTES antibody using peroxidase antiperoxidase method revealed constitutive staining in airway epithelial cells, airway smooth muscle and subepithelial cells. However, there was no difference in the number of RANTES-staining cells between normal subjects and asthmatics despite significantly increased numbers of CD4(+) T-cells, CD68(+) macrophages, and MBP(+) eosinophils in mucosal biopsies obtained from asthmatics. Double-staining revealed expression of RANTES in proportion of CD3(+) T lymphocytes. Thus, RANTES is constitutively expressed in the airways and RANTES mRNA is elevated in airways of patients with mild asthma, supporting a role for RANTES in normal and asthmatic airways.