ETHANOL AUGMENTS INTRACELLULAR SURVIVAL OF MYCOBACTERIUM-AVIUM COMPLEX AND IMPAIRS MACROPHAGE RESPONSES TO CYTOKINES
ETHANOL AUGMENTS INTRACELLULAR SURVIVAL OF MYCOBACTERIUM-AVIUM COMPLEX AND IMPAIRS MACROPHAGE RESPONSES TO CYTOKINES
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DOI：
10.1093/infdis/163.6.1286
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发表时间：
1991-06-01
影响因子：
6.4
通讯作者：
YOUNG, LS
中科院分区：
文献类型：
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作者：
BERMUDEZ, LE;YOUNG, LS
长期摄入乙醇容易患结核病和细菌性肺炎。 鸟分枝杆菌复合体 (MAC) 微生物会导致艾滋病患者出现菌血症。 将培养的人单核细胞来源的巨噬细胞和鼠 Kupffer 细胞暴露于 10-100-mu-g/dl 乙醇；与对照组相比，经乙醇处理的细胞中 MAC 菌株 100（血清型 8）和 101（血清型 1）的细胞内生长显着增加（范围为 58% +/- 7%-70% +/- 5%；与对照组相比，50 和 100-mu-g/dl 乙醇的 P < .05）。 两种细胞类型，在 10-100-mu-g/dl 乙醇存在下，用 10(3) 单位/ml 重组肿瘤坏死因子 (TNF) 或 10(2) 单位/ml 粒细胞巨噬细胞集落刺激因子 (GM-CSF) 处理时，杀死的 MAC 明显少于对照组（GM-CSF 减少 49% +/- 12%，TNF 减少 57% +/- 16%；P <所有比较均为 .05）。 静脉内感染 MAC 101 株的 C57BL 黑小鼠每天给予总热量 4% 的乙醇； 21 天后，他们的血液、肝脏和脾脏中的 MAC 数量比对照组多。 乙醇对宿主和 MAC 之间相互作用的影响有利于进行性感染。
Chronic ethanol ingestion predisposes to tuberculosis and bacterial pneumonia. Mycobacterium avium complex (MAC) organisms cause bacteremia in patients with AIDS. Cultured human monocyte-derived macrophages and murine Kupffer cells were exposed to 10-100-mu-g/dl ethanol; significantly greater intracellular growth of MAC strains 100 (serovar 8) and 101 (serovar 1) occurred in ethanol-treated cells than in controls (range, 58% +/- 7%-70% +/- 5%; P < .05 for 50 and 100-mu-g/dl ethanol vs. control). Both cell types, when treated with 10(3) units/ml recombinant tumor necrosis factor (TNF) or 10(2) units/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) in the presence of 10-100-mu-g/dl ethanol, killed significantly fewer MAC than controls (49% +/- 12% decrease for GM-CSF and 57% +/- 16% for TNF; P < .05 for all comparisons). C57BL black mice infected intravenously with MAC strain 101 were given ethanol as 4% of total calories daily; after 21 days they had greater numbers of MAC in blood, liver, and spleen than controls. Ethanol's effects on the interaction between the host and MAC favor progressive infection.