Release and spread of Wingless is required to pattern the proximo-distal axis of Drosophila renal tubules.
Release and spread of Wingless is required to pattern the proximo-distal axis of Drosophila renal tubules.
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DOI：
10.7554/elife.35373
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发表时间：
2018-08-10
期刊：
影响因子：
7.7
通讯作者：
Denholm B
中科院分区：
文献类型：
--
作者：
Beaven R;Denholm B
Wingless/Wnt是信号分子，传统上被认为是将组织模式化为长距离形态发生素。然而，最近的研究表明，在果蝇和脊椎动物的不同发育背景下，无翼的传播是不稳定的。在这里，我们证明了释放和传播的无翅是必需的模式的近端远端（PD）轴的果蝇马氏管。无翅的信号，从中肠发出，直接激活奇数跳过表达几个细胞远在近端小管。用不能从Wingless产生细胞扩散的膜系版本替换Wingless导致Malpighian小管P-D轴的异常模式和短的变形输尿管的发育。这项工作直接证明了无翼信号的模式化作用。除了扩展我们对Wnt塑造动物发育的功能模式的理解外，我们预计这种机制与其他器官（如脊椎动物肾脏）中的上皮管图案有关。
Wingless/Wnts are signalling molecules, traditionally considered to pattern tissues as long-range morphogens. However, more recently the spread of Wingless was shown to be dispensable in diverse developmental contexts in Drosophila and vertebrates. Here we demonstrate that release and spread of Wingless is required to pattern the proximo-distal (P-D) axis of Drosophila Malpighian tubules. Wingless signalling, emanating from the midgut, directly activates odd skipped expression several cells distant in the proximal tubule. Replacing Wingless with a membrane-tethered version that is unable to diffuse from the Wingless producing cells results in aberrant patterning of the Malpighian tubule P-D axis and development of short, deformed ureters. This work directly demonstrates a patterning role for a released Wingless signal. As well as extending our understanding about the functional modes by which Wnts shape animal development, we anticipate this mechanism to be relevant to patterning epithelial tubes in other organs, such as the vertebrate kidney.