Extrathymic development of V alpha 14-positive T cells
Extrathymic development of V alpha 14-positive T cells
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V α 14 阳性 T 细胞的胸腺外发育
DOI：
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发表时间：
1993
影响因子：
15.3
通讯作者：
Masaru Taniguchi
中科院分区：
文献类型：
--
作者：
Y. Makino;Nobuyuki Yamagata;Takahisa Sasho;Yoshihiro Adachi;R. Kanno;Haruhiko Koseki;Masamoto Kanno;Masaru Taniguchi
众所周知，T细胞抗原受体(TCR)基因重排发生在T细胞发育过程中的胸腺，导致可变(V)和多样性/连接片段之间的DNA缺失，并形成具有重组信号序列的环状DNA。在这里，我们提供的证据是Vα14+TCR基因重排发生在胸腺外部位，如骨髓、肝脏和肠道，而不是在脾，因为我们能够检测到胸腺外部位TCR重排导致的频繁的生产性和非生产性Vα14+编码和信号序列。在无瘤小鼠身上也发现了类似的发现。定量分析表明，胸腺外组织中Vα14基因介导的信号序列的相对数量高于胸腺组织。而胸腺中的Vα1.1T细胞TCR重排主要见于胸腺、Peyer‘s结和脾，而胸腺外其他组织中未见，表现出不同于Vα14TCR重排的模式。这些发现是胸腺外Vα14+T细胞发育的证据。不同特征的TCR重排模式也表明在不同的淋巴组织中产生了不同的TCR谱系。
It is known that rearrangement of the T cell antigen receptor (TCR) gene occurs in the thymus during T cell development and consequently results both in the deletion of DNA between the variable (V) and diversity/joining segments and in the formation of a circular DNA with recombination signal sequences. Here, we provide evidence that V alpha 14+ TCR gene rearrangements take place in extrathymic sites, such as bone marrow, liver, and intestine, but not in spleen, because we were able to detect frequent productive and nonproductive V alpha 14+ coding and signal sequences as a result of TCR rearrangements in extrathymic sites. Similar findings were also detected in athymic mice. Quantitative analysis shows that the relative amounts of V alpha 14 gene-mediated signal sequences in extrathymic tissues are higher than those in thymus. On the contrary, TCR rearrangements of V alpha 1.1 T cells, which are known to develop in the thymus, were mainly detected in the thymus, Peyer's patch, and spleen, but not in other extrathymic tissues, showing patterns distinct from V alpha 14 TCR rearrangements. These findings are evidence of extrathymic development of V alpha 14+ T cells. Differential characteristic TCR rearrangement patterns also indicate that distinct TCR repertoires are generated in different lymphoid tissues.
裸鼠中的 Thy-1 表皮细胞与胸腺小鼠中的对应物不同。
DOI：
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发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Nixon-Fulton,JL;Kuziel,WA;Santerse,B;Bergstresser,PR;Tucker,PW;Tigelaar,RE
通讯作者：
Tigelaar,RE
影响因子：
56.9
作者：
OETTINGER, MA;SCHATZ, DG;BALTIMORE, D
通讯作者：
BALTIMORE, D