Analysis of physiological responses and expression profiling of boron transporter-like genes in response to excess boron in Populus russkii
Analysis of physiological responses and expression profiling of boron transporter-like genes in response to excess boron in Populus russkii
复制标题
俄罗斯杨对过量硼的生理反应及硼转运蛋白样基因表达谱分析
DOI：
10.1016/j.chemosphere.2019.02.130
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发表时间：
2019-06-01
期刊：
影响因子：
8.8
通讯作者：
Yao, Yinan
中科院分区：
文献类型：
--
作者：
Ou, Yongbin;Wu, Xiuli;Yao, Yinan
Poplars (Populus species) are tolerant to boron (B) toxicity and have phytoremediation potential in B-contaminated soils. However, the detoxification strategy is largely unknown. To screen the key B transporter-like (BOR-like) genes for B compartmentation, Populus russkii plants were exposed to different levels of excess B and the plant growth, physiological responses, B distribution, and the expression patterns of BOR-like genes were characterized. P. russkii showed moderate tolerance to excess B although the plant growth was inhibited. The enhanced proline level and well-regulated antioxidant defense system were associated with B tolerance in leaves. The B absorbed by plants was predominantly allocated to leaves. Ten BOR-like genes were identified and seven of them showed tissue-specific expression patterns. PrBOR7 was identified as an important BOR-like gene possibly involved in the export of B from leaf cytoplasm because it was expressed specifically in leaves and induced by excess B. Yeast experiment assays verified that PrBOR7 functions as an efflux-type transporter and strongly improved cell tolerance to excess B. The expression patterns of BOR-like genes highlight the diversity of the family members in P. russkii, and PrBOR7 has potential as a candidate gene for B detoxification. (C) 2019 Elsevier Ltd. All rights reserved.
