Suppression and function of X-linked lethal and sterile mutations in Caenorhabditis elegans.
Suppression and function of X-linked lethal and sterile mutations in Caenorhabditis elegans.
复制标题
秀丽隐杆线虫 X 连锁致死和不育突变的抑制和功能。
DOI：
10.1093/genetics/97.1.65
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发表时间：
1981
期刊：
影响因子：
3.3
通讯作者：
Herman,RK
中科院分区：
文献类型：
--
作者：
Meneely,PM;Herman,RK
我们已经扩大了我们收集的隐性致死和不育突变体在该地区的X染色体平衡mnDp 1（X;V），约15%的X连锁图谱，共54突变体。这些突变已被定位为20个重叠缺陷和5个X重复，并通过互补测试将其分配给24个基因。九个突变体是雌雄同体不育的：其中一个是精子缺陷突变体，两个有异常的性腺发生和六个，在五个基因，是受母体影响的突变体，产生不活的合子后代。其中一个性腺发生突变体和两个受母体影响的突变体是雄性可育的。除一种受母体影响的突变体外，所有突变体在与野生型雄性交配时都会产生杂交后代。43个突变体进行了测试抑制homoadhesioussup-5（e1464），这被认为是特定的无效等位基因。通过独立标准判断不是无效突变体的10个突变体未被抑制。其他33个突变体中的9个，在9个基因中，被抑制，5个在杂合和纯合的抑制基因原种中，4个仅在纯合的抑制基因原种中。
We have expanded our collection of recessive lethal and sterile mutants in the region of theXchromosome balanced bymnDp1(X;V), about 15% of theXlinkage map, to a total of 54 mutants. The mutations have been mapped with respect to 20 overlapping deficiencies and fiveXduplications, and they have been assigned to 24 genes by complementation testing. Nine mutants are hermaphrodite-sterile: one of these is a sperm-defect mutant, two have abnormal gonadogeneses and six, in five genes, are maternally influenced mutants, producing inviable zygote progeny. One of the gonadogenesis mutants and two of the maternally influenced mutants are male fertile. All but one of the maternally influenced mutants give cross progeny when mated with wild-type males. Forty-three mutants were tested for suppression by homozygoussup-5(e1464), which is believed to be specific for null alleles. Ten mutants that were judged by independent criteria not to be null mutants are not suppressed. Nine of the other 33 mutants, in nine genes, are suppressed, five in both heterozygous and homozygous suppressor stocks and four only in homozygous suppressor stocks.