Interactions of Lipid Multilayers in the Presence of ATP
Interactions of Lipid Multilayers in the Presence of ATP
复制标题
ATP 存在下脂质多层的相互作用
DOI：
10.1016/j.bpj.2017.11.2004
复制
发表时间：
2018
影响因子：
3.4
通讯作者：
Petrache, Horia I.
中科院分区：
文献类型：
--
作者：
Lybarger, Ryan Z.;Costantino, Michele;Ramkumar, Abhinav;Ray, Bruce D.;Petrache, Horia I.
一类有趣的生物材料是磷脂双分子层的多层堆叠。在生物膜中发现的磷脂（简称脂类）由于其独特的机械和电学性质，在材料研究中具有重要的意义。在水或缓冲溶液中，脂质自发形成厚度为4-5纳米的膜结构。此外，范德华引力使膜形成有规则的叠层，这些叠层具有数十到数百纳米的重复晶格间距（d -间距）。d -间距取决于脂质类型和形成膜的缓冲溶液的组成。三磷酸腺苷（ATP）是一种在生物过程中参与能量传递的分子，因此是生物启发材料研究的兴趣所在。利用三种互补的实验方法，即小角x射线散射（SAXS）、核磁共振光谱（NMR）和动态光散射（DLS），以及分子动力学模拟（MD），我们证明了ATP可以用来修饰脂质堆的材料性质。特别是，ATP诱导从多层到单层的不结合转变，随着ATP浓度的增加，多层结构发生重整。ADP和AMP浓度逐渐升高时，也可观察到这种效应。这些发现可以帮助设计ATP及其水解产物控制层状结构材料性质的应用。
One interesting class of biomaterials are multilamellar stacks of phospholipid bilayers. Phospholipids (lipids in short) found in biological membranes are of significant interest in material research due to their distinctive mechanical and electrical properties. In water or buffer solutions, lipids spontaneously form membrane structures with thicknesses on the order of 4-5 nm. In addition, van der Waals attraction causes membranes to form regular stacks of many layers with repeat lattice spacings (D-spacings) on the order of tens to hundreds of nanometers. The D-spacing depends on both lipid type and on the composition of the buffer solution in which membranes are formed. Adenosine triphosphate (ATP) is a molecule involved in energy transfer in biological processes and is therefore of interest in bio-inspired material research. Using three complementary experimental methods, namely small-angle x-ray scattering (SAXS), NMR spectroscopy, and dynamic light scattering (DLS), as well as molecular dynamics simulations (MD), we show that ATP can be used to modify the material properties of lipid stacks. In particular, ATP induces an ubinding transition from multilamellar to single layers followed by reforming of multilayer structures as ATP concentration is increased. This effect is also seen with ADP and AMP at progressively higher concentrations. These findings can help design applications in which ATP and its hydrolysis products can control material properties of layered structures.