Filamentation of Metabolic Enzymes in Saccharomyces cerevisiae.
Filamentation of Metabolic Enzymes in Saccharomyces cerevisiae.
复制标题
酿酒酵母代谢酶的丝状化
DOI：
10.1016/j.jgg.2016.03.008
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发表时间：
2016-06-20
期刊：
影响因子：
--
通讯作者：
Liu JL
中科院分区：
文献类型：
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作者：
Shen QJ;Kassim H;Huang Y;Li H;Zhang J;Li G;Wang PY;Yan J;Ye F;Liu JL
通过纤维形成的区隔是最近出现的一种新的代谢调节机制。为了系统地鉴定成丝代谢酶，我们对酿酒酵母（Saccharomyces cerevisiae）的开放阅读框- gfp收集的所有菌株进行了全基因组筛选。我们发现了9种新的成丝蛋白，并证实了之前发现的蛋白。从4159个菌株中，我们发现了23种能够在体内形成细丝的蛋白质，其中大部分是代谢酶。硅蛋白-蛋白相互作用分析表明，这些成丝蛋白可以聚为几组，包括翻译起始机制和葡萄糖和氮代谢途径。以谷氨酰胺利用酶为例，我们发现培养条件影响代谢丝的发生和长度。此外，我们发现两种CTP合成酶（Ura7p和Ura8p）和两种天冬酰胺合成酶（Asn1p和Asn2p）在细胞质和细胞核中都形成细丝。实时成像分析表明，代谢丝经历了亚扩散。综上所述，我们的全基因组筛选鉴定了酿酒酵母中额外的丝状形成蛋白，并表明代谢酶的丝状形成比目前所认识的更为普遍。
Compartmentation via filamentation has recently emerged as a novel mechanism for metabolic regulation. In order to identify filament-forming metabolic enzymes systematically, we performed a genome-wide screening of all strains available from an open reading frame-GFP collection in Saccharomyces cerevisiae. We discovered nine novel filament-forming proteins and also confirmed those identified previously. From the 4159 strains, we found 23 proteins, mostly metabolic enzymes, which are capable of forming filaments in vivo. In silico protein-protein interaction analysis suggests that these filament-forming proteins can be clustered into several groups, including translational initiation machinery and glucose and nitrogen metabolic pathways. Using glutamine-utilising enzymes as examples, we found that the culture conditions affect the occurrence and length of the metabolic filaments. Furthermore, we found that two CTP synthases (Ura7p and Ura8p) and two asparagine synthetases (Asn1p and Asn2p) form filaments both in the cytoplasm and in the nucleus. Live imaging analyses suggest that metabolic filaments undergo sub-diffusion. Taken together, our genome-wide screening identifies additional filament-forming proteins in S. cerevisiae and suggests that filamentation of metabolic enzymes is more general than currently appreciated.