RELATIONSHIP BETWEEN DESICCATION AND EXOPOLYSACCHARIDE PRODUCTION IN A SOIL PSEUDOMONAS SP
RELATIONSHIP BETWEEN DESICCATION AND EXOPOLYSACCHARIDE PRODUCTION IN A SOIL PSEUDOMONAS SP
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DOI：
10.1128/aem.58.4.1284-1291.1992
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发表时间：
1992-04-01
影响因子：
4.4
通讯作者：
FIRESTONE, MK
中科院分区：
文献类型：
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作者：
ROBERSON, EB;FIRESTONE, MK
以一株分离自土壤的假单胞菌为材料，研究了土壤干燥与细菌胞外多糖（EPS）产生的关系。文化进行干燥，而生长在砂基质中含有更多的EPS和更少的蛋白质比那些生长在高水势，这表明资源分配到EPS生产响应干燥。干燥没有显着的影响，如通过减少碘硝基四唑测定的活性。在低水势下，假单胞菌培养物产生的纯化EPS在水中含有数倍于其重量的EPS。与未修正的沙子相比，用EPS修正的沙子保持了显着更多的水和干燥显着更慢，这意味着EPS基质可以缓冲细菌菌落的干燥的一些影响。我们的结论是，细菌可能使用EPS生产改变他们的微环境，以提高生存的干燥。
The relationship between desiccation and the production of extracellular polysaccharides (EPS) by soil bacteria was investigated by using a Pseudomonas species isolated from soil. Cultures subjected to desiccation while growing in a sand matrix contained more EPS and less protein than those growing at high water potential, suggesting that resources were allocated to EPS production in response to desiccation. Desiccation did not have a significant effect on activity as measured by reduction of iodonitrotetrazolium. Purified EPS produced by the Pseudomonas culture contained several times its weight in water at low water potential. Sand amended with EPS held significantly more water and dried significantly more slowly than unamended sand, implying that an EPS matrix may buffer bacterial colonies from some effects of desiccation. We conclude that bacteria may use EPS production to alter their microenvironment to enhance survival of desiccation.