TuJ1 (class III β-tubulin) expression suggests dynamic redistribution of follicular dendritic cellsin lymphoid tissue
TuJ1 (class III β-tubulin) expression suggests dynamic redistribution of follicular dendritic cellsin lymphoid tissue
复制标题
DOI：
10.1016/j.ejcb.2004.11.001
复制
发表时间：
2005-03-01
影响因子：
6.6
通讯作者：
Lee, I
中科院分区：
文献类型：
--
作者：
Lee, S;Choi, K;Lee, I
滤泡树突状细胞(FDC)在B细胞的存活、增殖和向记忆细胞分化过程中发挥重要作用。这里。我们发现TuJ1(III类β-微管蛋白)在人淋巴组织的FDCs中强烈表达。自神经分化早期起，TuJ1就是中枢和外周神经系统神经元的标志物。在所有人淋巴组织中，FDCs均可在明暗区或生发中心广泛表达TuJ1蛋白。相比之下，CD21的表达相对集中在光区，提示TuJ1是一个比CD21谱更广的FDCs市场。除生发中心外，套层内散在分布有单个TuJ1表达细胞，模糊了FDC网络的边界。在人扁桃体中，隐窝上皮中也可见散在分布的单个TuJ1阳性细胞，提示FDCs在富含抗原的上皮细胞--套层中动态地重新分布。和生发中心。FDCs的这种迁移可能反映了其表面携带的各种抗原直接运输到生发中心的方式，并为粘膜相关淋巴组织中淋巴滤泡朝向上皮的极性提供了基础。培养的FDC的HK细胞也表达TuJ1。FDC对TO的表达提示它们可能具有神经系统的某些生物学特性。(C)2004年爱思唯尔股份有限公司。版权所有。
Follicular dendritic cells (FDCs) play central roles in the B cell survival, proliferation, and differentiation into memory cells. Here. we show that TuJ1 (class III beta-tubulin) is expressed strongly in FDCs of human lymphoid tissue. TuJ1 has been a marker of neurons In the central and peripheral nervous systems from the early stage of neural differentiation. FDCs expressed TuJ1 protein diffusely in both light and dark zones or germinal centers in all human lymphoid tissues. In contrast, CD21 expression was relatively concentrated to the light zone, suggesting that TuJ1 was a market for FDCs with broader spectrum than CD21. In addition to the germinal center, there were single TuJ1-expressing cells scattered in the mantle zone, blurring the border of the FDC network. In human tonsils, single scattered TuJ1-positive cells were also present in the crypt epithelium, suggesting a dynamic redistribution of FDCs among the antigen-rich epithelium, mantle zone.. and germinal center. Such migration of FDCs could reflect a way of direct transport of various antigens carried on their surface to the germinal center, and a basis for the polarity of lymphoid follicles toward the epithelium in mucosa-associated lymphoid tissues. HK cells, cultured FDCs, also expressed TuJ1. The expression of TO by FDCs suggests that they may share certain biological characteristics of the neural system. (c) 2004 Elsevier GmbH. All rights reserved.