The odd one out: Bacillus ACT bacteriophage CP-51 exhibits unusual properties compared to related Spounavirinae W.Ph. and Bastille.
The odd one out: Bacillus ACT bacteriophage CP-51 exhibits unusual properties compared to related Spounavirinae W.Ph. and Bastille.
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DOI：
10.1016/j.virol.2014.06.012
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发表时间：
2014-08
期刊：
影响因子：
3.7
通讯作者：
J. Klumpp;Martina Schmuki;S. Sozhamannan;W. Beyer;D. Fouts;Vanessa Bernbach;R. Calendar;M. Loessner
中科院分区：
文献类型：
--
作者：
J. Klumpp;Martina Schmuki;S. Sozhamannan;W. Beyer;D. Fouts;Vanessa Bernbach;R. Calendar;M. Loessner
BacillusACT组包括三种重要的病原芽孢杆菌：炭疽芽孢杆菌、蜡状芽孢杆菌和苏云金芽孢杆菌。我们的特点是三个有毒的噬菌体，巴士底狱，W. Ph。和CP-51，感染这三个物种的各种菌株。我们已经测定了CP-51、W. Ph和Bastille的全基因组序列，以及它们的物理基因组结构。CP-51基因组序列只能使用传统的第二代和第三代测序技术的组合来获得，这说明了与测序高度修饰的DNA相关的问题。我们目前的证据表明，CP-51促进的广义转导是独立的特定的基因组结构，但可能是由于零星的包装错误的末端酶。这些病毒的遗传和形态特征之间存在明显的相关性，证实了它们在Myoviridae的Spounavirinaesubfamily（SPO 1-related spirinaesubfamily）中的位置。这项研究还为开发这组病原体的基于噬菌体的诊断/治疗提供了工具。
TheBacillusACT group includes three important pathogenic species ofBacillus:anthracis,cereusandthuringiensis. We characterized three virulent bacteriophages, Bastille, W.Ph. and CP-51, that infect various strains of these three species. We have determined the complete genome sequences of CP-51, W.Ph. and Bastille, and their physical genome structures. The CP-51 genome sequence could only be obtained using a combination of conventional and second and third next generation sequencing technologies – illustrating the problems associated with sequencing highly modified DNA. We present evidence that the generalized transduction facilitated by CP-51 is independent of a specific genome structure, but likely due to sporadic packaging errors of the terminase. There is clear correlation of the genetic and morphological features of these phages validating their placement in theSpounavirinaesubfamily (SPO1-related phages) of theMyoviridae. This study also provides tools for the development of phage-based diagnostics/therapeutics for this group of pathogens.