GATAe-dependent and -independent expressions of genes in the differentiated endodermal midgut of Drosophila
GATAe-dependent and -independent expressions of genes in the differentiated endodermal midgut of Drosophila
复制标题
DOI：
10.1016/j.modgep.2006.07.001
复制
发表时间：
2007-01-01
影响因子：
1.2
通讯作者：
Murakami, Ryutaro
中科院分区：
文献类型：
--
作者：
Okumura, Takashi;Tajiri, Reiko;Murakami, Ryutaro
两个序列表达的GATA因子基因serpent （srp）和GATAe对果蝇内胚层的发育至关重要。最早的内胚层GATA基因srp被认为指定内胚层命运，激活第二个GATA基因GATAe，后者在内胚层中肠中持续表达一生。先前，我们提出GATAe建立并维持中肠的终末分化状态，因为中肠中的一些功能基因需要GATAe活性才能表达。为了获得GA tae作用的进一步证据，我们寻找了在中肠晚期特异性表达的其他基因，并检查了总共选择的15个基因对GATAe耗尽和过表达的反应。15个基因中有10个基因在缺乏GATAe活性的胚胎中表达失败，而其他5个基因不需要GATAe。相反，srp是激活这五个基因所必需的。这些观察结果表明，GATAe激活了中肠中一个主要的基因亚群，而srp下游的一些其他途径激活了其他基因。(c) 2006 Elsevier B.V.版权所有
Two sequentially-expressed GATA factor genes, serpent (srp) and GATAe, are essential for development of the Drosophila endoderm. The earliest endodermal GATA gene, srp, has been thought to specify the endodermal fate, activating the second GATA gene GATAe, and the latter continues to be expressed in the endodermal midgut throughout life. Previously, we proposed that GATAe establishes and maintains the state of terminal differentiation of the midgut, since some functional genes in the midgut require GATAe activity for their expression. To obtain further evidence of the role of GA TA e, we searched for additional genes that are expressed specifically in the midgut in late stages, and examined responses of a total of selected 15 genes to the depletion and overexpression of GATAe. Ten of the 15 genes failed to be expressed in the embryo deficient for GATAe activity, but, the other five genes did not require GATAe. Instead, srp is required for activating the five genes. These observations indicate that GATAe activates a major subset of genes in the midgut, and some other pathway(s) downstream of srp activates other genes. (c) 2006 Elsevier B.V. All rights reserved.