WATER FLOW THROUGH FROG GASTRIC MUCOSA
WATER FLOW THROUGH FROG GASTRIC MUCOSA
复制标题
DOI：
10.1085/jgp.39.4.535
复制
发表时间：
1956-01-01
影响因子：
3.8
通讯作者：
SOLOMON, AK
中科院分区：
文献类型：
--
作者：
DURBIN, RP;FRANK, H;SOLOMON, AK
对青蛙离体胃粘膜的水输送进行了实验。 (1)使用氚化水作为示踪剂的单向扩散流为180μl/cm 2 小时。 (2)通过将粘膜分泌侧的正常渗透压加倍，产生15μl/cm 2 /小时的渗透净流量，其体积与自发分泌的体积相当。使用精确至±2μl的体积室进行净流量测量。对膜对各种尺寸分子和离子扩散的限制的测量表明，93%的孔面积由2.5 A半径的孔贡献，7%由60 A半径的孔贡献。
Experiments were performed on water transport across the isolated gastric mucosa of the frog. (1) The unidirectional diffusion flow using tritiated water as a tracer was 180 [mu]l/cm2 hour. (2) An osmotic net flow of 15 [mu]l/cm2/hour, comparable in volume with that spontaneously secreted, was produced by doubling the normal osmolarity on the secretory side of the mucosa. Net flow measurements were made using a volume chamber accurate to [plus or minus] 2 [mu]l. Measurements of the restriction offered by the membrane to diffusion of various sized molecules and ions indicate 93% of the pore area was contributed by pores of 2.5 A radius and 7% by pores of 60 A radius.