Biosynthesis of glycosphingolipids by mouse neuroblastoma (NB41A), rat glia (RGC-6) and human glia (CHB-4) in cell culture.
Biosynthesis of glycosphingolipids by mouse neuroblastoma (NB41A), rat glia (RGC-6) and human glia (CHB-4) in cell culture.
复制标题
小鼠神经母细胞瘤 (NB41A)、大鼠神经胶质细胞 (RGC-6) 和人神经胶质细胞 (CHB-4) 在细胞培养物中生物合成鞘糖脂。
DOI：
10.1016/s0006-291x(71)80138-9
复制
发表时间：
1971
影响因子：
3.1
通讯作者：
J. Kennedy
中科院分区：
文献类型：
--
作者：
G. Dawson;S. Kemp;A. Stoolmiller;A. Dorfman;J. Kennedy
一种小鼠神经母细胞瘤细胞系被发现合成至少四种神经组织特有的神经节苷脂，GM 2，GM 1，GD 2和GD 1a。由于神经母细胞瘤细胞系中几乎不存在血苷（GM 3），因此数据表明，无唾液酸-GM 2是神经节苷脂合成和催化的中间体。大鼠和人类来源的神经胶质肿瘤细胞系主要含有GM 3（总糖脂含量的90%）和可忽略不计的含己糖胺的鞘糖脂。
A mouse neuroblastoma cell line was found to synthesize at least four of the gangliosides characteristic of nervous tissue, GM2, GM1, GD2and GD1a. Since hematoside (GM3) is virtually absent from the neuroblastoma cell line, the data suggest that asialo-GM2is an intermediate in the synthesis and catabolism of gangliosides. Glial tumor cell lines of both rat and human origin contained mainly GM3(90% of the total glycolipid content) and negligible amounts of hexosamine-containing glycosphingolipids.