Antioxidant Effects of Apocynum venetum Tea Extracts on d-Galactose-Induced Aging Model in Mice
Antioxidant Effects of Apocynum venetum Tea Extracts on d-Galactose-Induced Aging Model in Mice
复制标题
罗布麻茶提取物对D-半乳糖衰老小鼠抗氧化作用的研究
DOI：
10.3390/antiox8090381
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发表时间：
2019-09-01
期刊：
影响因子：
7
通讯作者：
Zhao, Xin
中科院分区：
文献类型：
--
作者：
Li, Chong;Tan, Fang;Zhao, Xin
罗布麻（Apocynum venetum）是中国新疆的一种传统药用饮料，因其浓郁的风味和生物功能而备受青睐。以d-半乳糖致衰老小鼠为实验对象，研究罗布麻茶提取物及其生物活性成分的体内抗衰老作用。我们评估了主要器官的重量（通过器官指数）和肝脏的病理变化。我们还详细介绍了AVTE（低剂量组250 mg/kg，高剂量组500 mg/kg）对衰老小鼠血清生化参数（丙二醛、超氧化物歧化酶、谷胱甘肽、谷胱甘肽过氧化物酶、过氧化氢酶、总抗氧化能力和一氧化氮）和细胞因子（IL-6、IL-12、tnf - α和IL-1 β）的影响。通过实时定量逆转录聚合酶链反应（RT-qPCR），研究AVTE对d-半乳糖诱导衰老小鼠的抗衰老作用。此外，我们还利用高效液相色谱（HPLC）分析了AVTEs的生物成分。结果非常显著，表明AVTE对d-半乳糖诱导的衰老小鼠有显著改善作用，其中高剂量组效果最好。ATVE可有效缓解小鼠肝细胞水肿、炎症细胞浸润和损伤，通过上调谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH- px）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）抗氧化相关因子诱导保护作用，并在上调抗炎因子（IL-10）、下调促炎因子（IL-6、tnf - α、IL-1 β）中发挥重要作用。同时，HPLC分析表明，AVTEs中含有新绿原酸、绿原酸、隐绿原酸、芦丁、异槲皮苷、异绿原酸B、异绿原酸A、黄芪甲苷、异绿原酸C、迷迭香酸和反式肉桂酸。因此，由于其丰富的功能成分和抗衰老活性，AVTE似乎是一种有效的功能性饮料。
As a traditional Chinese medicinal drink, Apocynum venetum, a local tea from Xinjiang, China, is favored for its rich flavor and biological functionality. This study looked at aging mice induced by d-galactose to determine the in vivo anti-aging effect of Apocynum venetum tea extracts (AVTEs) and its bioactive components. We evaluated the weight of major organs (via organ index) and pathological changes in the liver. We also detailed the effects of AVTE (250 mg/kg in the low dose group, 500 mg/kg in the high dose group) on biochemical parameters (malondialdehyde, superoxide dismutase, glutathione, glutathione peroxidase, catalase, total antioxidant capacity, and nitric oxide) and cytokines (IL-6, IL-12, TNF-alpha and IL-1 beta) in the serum of aging mice. We investigated the anti-aging effects of AVTE in d-galactose-induced aging mice via quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) assay. In addition, we analyzed the biological components of AVTEs by high performance liquid chromatography (HPLC). The results were remarkable, suggesting that AVTE significantly improved d-galactose-induced aging mice, with the high dose group showing the best results among other groups. ATVE can effectively alleviate hepatocyte edema, as well as inflammatory cell infiltration and injury in mice, induce a protective effect via up-regulation of glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) antioxidant related factors, and play an important role in the up-regulation of anti-inflammatory factors (IL-10) and the down-regulation of pro-inflammatory factors (IL-6, TNF-alpha and IL-1 beta). At the same time, HPLC analysis showed that AVTEs contain neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, isoquercitrin, isochlorogenic acid B, isochlorogenic acid A, astragalin, isochlorogenic acid C, rosmarinic acid, and trans-cinnamic acid. Thus, AVTE appears to be an effectively functional drink due to its rich functional components and anti-aging activities.