EVIDENCE THAT OPIATERGIC AND α-ADRENERGIC MECHANISMS STIMULATE RAT GROWTH HORMONE-RELEASE VIA GROWTH RELEASING FACTOR (GRF)
EVIDENCE THAT OPIATERGIC AND α-ADRENERGIC MECHANISMS STIMULATE RAT GROWTH HORMONE-RELEASE VIA GROWTH RELEASING FACTOR (GRF)
复制标题
有证据表明阿片剂和 α-肾上腺素能机制通过生长释放因子 (GRF) 刺激大鼠生长激素释放
DOI：
10.1210/endo-114-5-1950
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发表时间：
1984
期刊：
影响因子：
4.8
通讯作者：
K. Shizume
中科院分区：
文献类型：
--
作者：
N. Miki;M. Ono;K. Shizume
用人胰腺生长激素释放因子-（1-40）（hpGRF-40）的抗血清识别大鼠下丘脑生长激素释放因子（rhGRF），该抗血清能完全阻断rhGRF（0.2，1 μg/kg BW）诱导的GH分泌，并能抑制生理性GH分泌2天以上。用抗hpGRF-40血清被动免疫后，静脉注射FK 33-824（100 μg/kg BW）（一种脑啡肽类似物）或可乐定（125 μg/kg BW）（一种α-肾上腺素能药物）均不能刺激未麻醉大鼠的GH释放。这些结果表明，阿片能和α-肾上腺素能机制的GH释放作用是通过刺激大鼠内源性rhGRF释放介导的。
An antiserum raised against human pancreatic growth hormone-releasing factor-(1–40) (hpGRF-40) was found to recognize the rat hypothalamic growth hormone-releasing factor (rhGRF): This antiserum, when given iv to adult male rats, completely abolished GH release induced by a synthetic rhGRF (0.2, 1 μg/kg BW), and inhibited the physiological GH secretion over 2 days. After passive immunization with anti-hpGRF-40 serum, iv injection of either FK 33–824 (100 μg/kg BW), an enkephalin analogue, or clonidine (125 μg/kg BW), an α-adrenergic agent, failed to stimulate GH release in the unanesthetized rat. These results indicate that the GHreleasing actions of opiatergic and a-adrenergic mechanisms are mediated through stimulation of endogenous rhGRF release in the rat.