Novel Caspase-Suicide Proteins for Tamoxifen-Inducible Apoptosis
Novel Caspase-Suicide Proteins for Tamoxifen-Inducible Apoptosis
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DOI：
10.1002/dvg.20426
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发表时间：
2008-10-01
期刊：
影响因子：
1.5
通讯作者：
Kuehn, Ralf
中科院分区：
文献类型：
--
作者：
Chu, Yuanyuan;Senghaas, Niklas;Kuehn, Ralf
利用突变的雌激素受体配体结合域(ERT)，我们开发了可诱导细胞凋亡的新型Caspase融合蛋白。我们发现Caspase-ERT2融合蛋白可以被合成配体4-OH-Tamoxifen特异性激活，并迅速诱导人、小鼠和斑马鱼细胞的凋亡细胞死亡。这种新的靶向细胞消融工具极大地促进了疾病模型的生成以及模型生物的发育和再生研究。创世纪46：530-536,2008。(C)2008年Wiley-Liss，Inc.
Taking advantage of a mutant estrogen receptor ligand binding domain (ER T), we developed novel Caspase fusion proteins for inducible apoptosis. We show that Caspase-ERT2 fusion proteins become specifically activated by the synthetic ligand 4-OH-tamoxifen and rapidly induce apoptotic cell death in human, murine, and zebrafish cells. This novel tool for targeted cell ablation greatly facilitates the generation of disease models as well as developmental and regeneration studies in model organisms. genesis 46:530-536, 2008. (C) 2008 Wiley-Liss, Inc.