Major histocompatibility complex loci are associated with susceptibility of Atlantic salmon to infectious hematopoietic necrosis virus
Major histocompatibility complex loci are associated with susceptibility of Atlantic salmon to infectious hematopoietic necrosis virus
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DOI：
10.1023/b:ebfi.0000022874.48341.0f
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发表时间：
2004-03-01
影响因子：
1.4
通讯作者：
Ming, TJ
中科院分区：
文献类型：
--
作者：
Miller, KM;Winton, JR;Ming, TJ
传染性造血坏死病毒(IHNV)是鲑鱼类最重要的病毒病原体之一，也是导致养殖鱼类死亡的主要原因。尽管已经开发了几种疫苗策略，其中一些是高度保护性的，但输送系统仍然过于昂贵，无法被水产养殖业普遍使用。更具成本效益的方法可能来自识别与IHNV抗性相关的基因，用于选择性育种。此外，识别易感基因可能有助于更好地了解病毒的发病机制，因此可能有助于制定预防和治疗措施。主要组织相容性复合体(MHC)基因参与获得性免疫反应中对外来病原体的初级识别，与脊椎动物对多种疾病的抗性有关。我们用三种不同剂量的IHNV对大西洋鲑鱼养殖品系进行了MHC病关联的初步分析，并用变性梯度凝胶电泳法对个体鱼的三个MHC基因座进行了基因分型，然后对所有分化的等位基因进行了测序。每个外显子座位有9到14个等位基因，并鉴定出可能与抗性或感病相关的等位基因。来自潜在的非经典I类基因座的一个等位基因(SASA-B-04)与感染性造血坏死的抵抗力高度相关(P<；0.01)。这些信息可用于设计特定单倍型的杂交，用于疾病关联的家系分析。
Infectious hematopoietic necrosis virus (IHNV) is one of the most significant viral pathogens of salmonids and is a leading cause of death among cultured juvenile fish. Although several vaccine strategies have been developed, some of which are highly protective, the delivery systems are still too costly for general use by the aquaculture industry. More cost effective methods could come from the identification of genes associated with IHNV resistance for use in selective breeding. Further, identification of susceptibility genes may lead to an improved understanding of viral pathogenesis and may therefore aid in the development of preventive and therapeutic measures. Genes of the major histocompatibility complex (MHC), involved in the primary recognition of foreign pathogens in the acquired immune response, are associated with resistance to a variety of diseases in vertebrate organisms. We conducted a preliminary analysis of MHC disease association in which an aquaculture strain of Atlantic salmon was challenged with IHNV at three different doses and individual fish were genotyped at three MHC loci using denaturing gradient gel electrophoresis (PCR-DGGE), followed by sequencing of all differentiated alleles. Nine to fourteen alleles per exon-locus were resolved, and alleles potentially associated with resistance or susceptibility were identified. One allele (Sasa-B-04) from a potentially non-classical class I locus was highly associated with resistance to infectious hematopoietic necrosis (p < 0.01). This information can be used to design crosses of specific haplotypes for family analysis of disease associations.