Isolation and characterization of two linked mouse U1b small nuclear RNA genes.
Isolation and characterization of two linked mouse U1b small nuclear RNA genes.
复制标题
两个相连的小鼠 U1b 小核 RNA 基因的分离和表征。
DOI：
10.1093/nar/11.18.6255
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发表时间：
1983
影响因子：
14.9
通讯作者：
Wang,SS
中科院分区：
文献类型：
--
作者：
Marzluff,WF;Brown,DT;Lobo,S;Wang,SS
已从小鼠DNA文库中分离出含有两个U1 RNA基因的6.9内切酶Eco R1片段。这两个基因编码的RNA与小鼠U1b RNA非常相似，如果不是相同的话，通过S1核酸酶图谱判断。这种RNA比小鼠U1a RNA、人U1 RNA和大鼠U1 RNA长一个碱基，与大鼠U1 RNA有六个核苷酸取代。这两个基因相隔5个碱基，U1 RNA编码在DNA的相反链上，5′端并置。基因侧翼序列与基因5′端有700个碱基相同，与基因3′端至少有80个碱基相同。
A 6.9 kilobase Eco R1 fragment containing genes for two U1 RNAs has been isolated from a library of mouse DNA. The two genes code for an RNA which is very similar, if not identical, to mouse U1b RNA as judged by S1 nuclease mapping. This RNA is one base longer than the mouse U1a RNA, human U1 RNA, and rat U1 RNA and differs in six nucleotide substitutions from rat U1 RNA. The two genes are five kilobases apart and the U1 RNAs are coded for on opposite strands of the DNA with the 5′ends juxtaposed. The sequences flanking the genes are identical for 700 bases 5′ to the gene and at least 80 bases 3′ to the gene.