Cloning and reproductive regulation of a trypsin precursor gene in Adelphocoris Suturalis.
Cloning and reproductive regulation of a trypsin precursor gene in Adelphocoris Suturalis.
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DOI：
10.1016/j.ijbiomac.2021.09.158
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发表时间：
2021-09
影响因子：
8.2
通讯作者：
Xingxing Huang;B. Zhu;Wei Zhang;Lizhen Chen
中科院分区：
文献类型：
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作者：
Xingxing Huang;B. Zhu;Wei Zhang;Lizhen Chen
Adelphocoris suturalisis 是棉花的主要害虫。在这里，我们在 A.suturali 中鉴定了胰蛋白酶前体基因 (AsTryP)，其开放阅读框区域为 873 bp，属于胰蛋白酶超家族。 AsTryP基因的mRNA在8日龄雌虫各个生命阶段和不同组织中均可检测到，且该基因在四龄若虫和8日龄雌虫胸部高表达。通过注射双链RNA下调AsTryP抑制卵巢发育和女性生育力。这些结果表明，胰蛋白酶前体参与了A.suturalis的繁殖过程，并且可能有助于开发更好地控制A.suturalis的新策略。
Adelphocoris suturalisis a major pest of cotton. Here, we identified atrypsin precursorgene (AsTryP) inA.suturali, which has an open reading frame region of 873 bp and belongs to the trypsin superfamily. The mRNA of theAsTryPgene was detectable in every life stage and different tissues of 8-day-old females, and the gene was highly expressed in fourth-instar nymphs and the thorax of 8-day-old females. Down-regulation ofAsTryPby the injection of double-stranded RNA suppressed the ovarian development and female fertility. These results reveal that trypsin precursor is involved in the reproductive process ofA.suturali, and may facilitate the development of new strategies for a better control ofA.suturalis.