HEMATOPOIETIC ENGRAFTMENT FOLLOWING TRANSPLANTATION OF BONE-MARROW CELLS CARRYING A PHILADELPHIA (PH')-LIKE CHROMOSOME
HEMATOPOIETIC ENGRAFTMENT FOLLOWING TRANSPLANTATION OF BONE-MARROW CELLS CARRYING A PHILADELPHIA (PH')-LIKE CHROMOSOME
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DOI：
10.1002/ajh.2830030204
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发表时间：
1977-01-01
影响因子：
12.8
通讯作者：
GALE, RP
中科院分区：
文献类型：
--
作者：
GRAZE, P;SPARKES, R;GALE, RP
一位表型正常的骨髓移植供体具有先前未报告的核型 46, XY, t(18q+; 22q-)，从而产生 Ph'' 样染色体。在皮肤成纤维细胞、植物凝集素刺激的外周血细胞和来自骨髓供体的骨髓细胞的培养物中鉴定出Ph''样染色体，但在兄弟姐妹或父母的细胞培养物中没有鉴定出Ph''样染色体，表明它代表了一种获得性体细胞突变。移植后骨髓移植受者的血液和骨髓细胞中Ph''样染色体的证明提供了独特且明确的移植标记。移植后，供体和白血病受体的造血功能似乎正常。这项研究表明，在造血细胞中产生 Ph'' 样染色体的突变不一定会产生血液学异常。这种易位的存在似乎不会干扰移植环境中正常的造血或淋巴分化和复制。
A phenotypically normal donor for bone marrow transplantation had a previously unreported karyotype, 46, XY, t(18q+; 22q-), resulting in a Ph''-like chromosome. Identification of the Ph''-like chromosome in cultures of skin fibroblasts, phytohemaglutinin-stimulated peripheral blood cells and bone marrow cells from the marrow donor, but not in cell cultures from siblings or parents, indicated that it represented an acquired somatic mutation. Demonstration of the Ph''-like chromosome in the marrow graft recipient''s blood and bone marrow cells after transplantation provided a unique and definitive marker of engraftment. Hematopoiesis appeared normal in the donor and leukemic recipient after transplantation. This study indicates that a mutation creating a Ph''-like chromosome in hematopoietic cells need not produce hematologic abnormality. The presence of this translocation did not appear to interfere with normal hematopoietic or lymphoid differentiation and replication in the transplant setting.