Identification of the Genetic Variation and Gene Exchange between Citrus Trifoliata and Citrus Clementina
Identification of the Genetic Variation and Gene Exchange between Citrus Trifoliata and Citrus Clementina
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柑橘与柑橘遗传变异及基因交换的鉴定
DOI：
10.3390/biom8040182
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发表时间：
2018-12-01
期刊：
影响因子：
5.5
通讯作者：
Hu, Chun-Gen
中科院分区：
文献类型：
--
作者：
Liu, Tian-Jia;Zhou, Jing-Jing;Hu, Chun-Gen
为了确定三叶柑橘和小柑橘之间的遗传变异，我们对这两种柑橘进行了基因组重测序。与柑橘参考基因组相比，两种柑橘共鉴定出9449204个单核苷酸多态性（snp）和846615个插入/缺失多态性（indel），其中1868115个（19.77%）snp和190199个（22.47%）indel位于基因区。同时，两种柑橘基因型共鉴定出8091407个特异性snp和692654个特异性InDels，平均产生27.32个snp /kb和2.34个InDels/kb。我们利用基因组重测序的特定遗传变异，鉴定并表征了嫁接柑橘植株的基因交换模式。共鉴定出4396个跨移植物连接的转运基因。Sanger测序还证实了一些特定的遗传变异和可移动基因。此外，这些移动基因可以在接穗和砧木之间进行定向或双向移动。嫁接后接穗和砧木中分别有1581个和2577个差异表达基因。这些遗传变异为三叶草和小红花之间重要性状的遗传基础提供了基础信息，基因的转运将适用于园艺作物。
To identify the genetic variation between Citrus trifoliata and Citrus clementina, we performed genome resequencing on the two citrus species. Compared with the citrus reference genome, a total of 9,449,204 single-nucleotide polymorphisms (SNPs) and 846,615 insertion/deletion polymorphisms (InDels) were identified in the two citrus species, while 1,868,115 (19.77%) of the SNPs and 190,199 (22.47%) of the InDels from the two citrus species were located in the genic regions. Meanwhile, a total of 8,091,407 specific SNPs and 692,654 specific InDels were identified in the two citrus genotypes, yielding an average of 27.32 SNPs/kb and 2.34 InDels/kb. We identified and characterized the patterns of gene exchanges in the grafted citrus plants by using specific genetic variation from genome resequencing. A total of 4396 transporting genes across graft junctions was identified. Some specific genetic variation and mobile genes was also confirmed by Sanger sequencing. Furthermore, these mobile genes could move directionally or bidirectionally between the scions and the rootstocks. In addition, a total of 1581 and 2577 differentially expressed genes were found in the scions and the rootstocks after grafting compared with the control, respectively. These genetic variations provide fundamental information on the genetic basis of important traits between C. trifoliata and C. clementina, as the transport of genes would be applicable to horticulture crops.