Generation of a monoclonal antibody specific for ganglioside GM4: evidence for GM4 expression on astrocytes in chicken cerebellum.
Generation of a monoclonal antibody specific for ganglioside GM4: evidence for GM4 expression on astrocytes in chicken cerebellum.
复制标题
神经节苷脂 GM4 特异性单克隆抗体的产生：鸡小脑星形胶质细胞 GM4 表达的证据。
DOI：
10.1093/oxfordjournals.jbchem.a124138
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发表时间：
1993
影响因子：
2.7
通讯作者：
T. Tai
中科院分区：
文献类型：
--
作者：
H. Ozawa;M. Kotani;I. Kawashima;M. Numata;T. Ogawa;T. Terashima;T. Tai
We established a murine monoclonal antibody (MAb) specific for ganglioside GM4 by immunizing C3H/HeN mice with chemically synthesized GM4 adsorbed to Salmonella minnesota, followed by fusion with mouse myeloma cells. The MAb, designated as AMR10, was shown to exhibit high binding specificity, reacting only with the ganglioside GM4 used for immunization and native GM4 from human brain. We determined the distribution of GM4 in adult chicken cerebellum by means of the immunofluorescence technique with the MAb. Our study revealed that GM4 expression was associated with astrocytes in the granular layer and the white matter, but not with myelin in any layers of the chicken cerebellar cortex.
