In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite
In vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite
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DOI：
10.1016/s1079-2104(03)00360-3
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
de Souza, FJ
中科院分区：
文献类型：
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作者：
Vianna, ME;Gomes, BPFA;de Souza, FJ
本研究的目的是研究0.2%，1%和2%葡萄糖酸氯己定（CHX凝胶和CHX液体）对牙髓病原体的体外抗菌活性，并与0.5%，1%，2.5%，4%和5.25%次氯酸钠（NaOCl）的结果进行比较。进行肉汤稀释试验，记录冲洗液杀死微生物细胞的时间并进行统计分析。2.0%凝胶和液体制剂均在15秒内消除金黄色葡萄球菌和白色念珠菌，而凝胶制剂在1分钟内杀死粪肠球菌。所有测试的冲洗剂在15秒内消除牙髓卟啉单胞菌、牙龈卟啉单胞菌和中间普氏菌。1.0%和2.0%CHX液体消除所有微生物所需的时间与5.25%NaOCl所需的时间相同。其抑菌作用与冲洗剂的种类、浓度、表现形式及微生物敏感性有关。
The aim of this study was to investigate in vitro the antimicrobial activity of 0.2%, 1%, and 2% chlorhexidine gluconate (CHX gel and CHX liquid), against endodontic pathogens and compare the results with the ones achieved by 0.5%, 1%, 2.5%, 4%, and 5.25% sodium hypochlorite (NaOCl). A broth dilution test was performed, and the timing for irrigants to kill microbial cells was recorded and statistically analyzed. Both 2.0% gel and liquid formulations eliminated Staphylococcus aureus and Candida albicans in 15 seconds, whereas the gel formulation killed Enterococcus faecalis in 1 minute. All tested irrigants eliminated Porphyromonas endodontalis, Porphyromonas gingivalis, and Prevotella intermedia in 15 seconds. The timing required for 1.0% and 2.0% CHX liquid to eliminate all microorganisms was the same required for 5.25% NaOCl. The antimicrobial action is related to type, concentration, and presentation form of the irrigants as well as the microbial susceptibility.