NUCLEOTIDE-SEQUENCE ADJOINING 3' END OF GENES CODING FOR OOCYTE-TYPE 5 S RIBOSOMAL-RNA IN XENOPUS
NUCLEOTIDE-SEQUENCE ADJOINING 3' END OF GENES CODING FOR OOCYTE-TYPE 5 S RIBOSOMAL-RNA IN XENOPUS
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DOI：
10.1016/0022-2836(76)90070-x
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发表时间：
1976-01-01
影响因子：
5.6
通讯作者：
BROWN, DD
中科院分区：
文献类型：
--
作者：
BROWN, RD;BROWN, DD
本文报道了非洲爪蟾（Xenopus laevis）和非洲爪蟾（Xenopus mulleri）卵母细胞特异性5S核糖体RNA编码基因3′端附近DNA的部分核苷酸序列。将5S RNA与编码它的分离的DNA链杂交，并用作通过大肠杆菌DNA聚合酶I进行DNA合成的引物。合成短的、核糖取代的延伸片段并测序。X.与有义链互补的5 S DNA为5′ 5S RNA 3′ TTCAAAGT。副艇长mulleri间隔区在含有基因TTA/CG（N）之后的序列的该区域内在序列上是异质的，其中N包括T、A和C以及可能的G。我们认为，终止RNA合成的重要序列可能是两个爪蟾物种之间的保守。如果这是真的，那么这个终止信号的一部分可能包括基因本身的两个3′末端核苷酸和间隔区中的前两个相邻核苷酸：（基因）5′ TTTT AAAA 3′（间隔区）。X染色体5S RNA基因3′端附近的核苷酸序列。乳杆菌5 S DNA类似于约15个核苷酸长的重复序列，其包含大部分富含（A+ T）的间隔区。
A partial nucleotide sequence has been obtained for the DNA adjacent to the 3′ end of the gene coding for oocyte-specific 5 S ribosomal RNA in Xenopus laevis and Xenopus mulleri. The 5 S RNA was hybridized to the isolated DNA strand that codes for it, and used as a primer for DNA synthesis by Escherichia coli DNA polymerase I. Short, ribo-substituted extension fragments were synthesized and sequenced. The sequence of X. laevis 5 S DNA complementary to the sense strand is 5′ 5S RNA 3′ TTCAAAGT. The X. mulleri spacer is heterogeneous in sequence within this region containing the sequences following the gene TTA/CG (N), where N includes T, A and C and possibly G. We suggest that sequences important for the termination of RNA synthesis are likely to be conserved between the two Xenopus species. If this is true, then part of this termination signal may include the two 3′ terminal nucleotides of the gene itself and the first adjacent two nucleotides in the spacer:(gene) 5′ TTTT AAAA 3′(spacer). The nucleotide sequence adjacent to the 3′ end of the 5 S RNA gene within X. laevis 5 S DNA resembles the approximately 15 nucleotide long reiterated sequences which comprise most of the (A+ T)-rich spacer region.