Left Pulmonary Artery Ligation and Chronic Pulmonary Artery Banding Model for Inducing Right Ventricular-Pulmonary Hypertension in Sheep.
Left Pulmonary Artery Ligation and Chronic Pulmonary Artery Banding Model for Inducing Right Ventricular-Pulmonary Hypertension in Sheep.
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DOI：
10.1097/mat.0000000000001197
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发表时间：
2021-01-01
期刊：
影响因子：
--
通讯作者：
Bacchetta M
中科院分区：
文献类型：
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作者：
Ukita R;Tipograf Y;Tumen A;Donocoff R;Stokes JW;Foley NM;Talackine J;Cardwell NL;Rosenzweig EB;Cook KE;Bacchetta M
肺动脉高压（PH）是一种进行性疾病，其由于右心室（RV）的压力和容量超负荷而导致心肺功能障碍和右心衰竭。机械心肺支持在理论上有望成为这些患者器官移植或目的地治疗的桥梁。解决PH和RV衰竭的机械心肺支持的挑战需要在生理相关的动物模型中进行测试。以前在大型动物中的PH模型使用了肺珠栓塞，其引起不可预测的炎症反应并且具有高死亡率。我们描述了通过左肺动脉（LPA）结扎结合渐进性主肺动脉（MPA）结扎诱导绵羊肺动脉高压和右心室肥大（PH-RVH）的分步指南。这种方法提供了一种受控的方法来调节RV后负荷，使其达到动物耐受的水平，以实现PH-RVH，同时降低急性死亡率。该动物模型可用于评估PH和RV衰竭的机械支持装置。
Pulmonary hypertension (PH) is a progressive disease that leads to cardiopulmonary dysfunction and right heart failure from pressure and volume overloading of the right ventricle (RV). Mechanical cardiopulmonary support has theoretical promise as a bridge to organ transplant or destination therapy for these patients. Solving the challenges of mechanical cardiopulmonary support for PH and RV failure requires its testing in a physiologically relevant animal model. Previous PH models in large animals have used pulmonary bead embolization, which elicits unpredictable inflammatory responses and has a high mortality rate. We describe a step-by-step guide for inducing pulmonary hypertension and right ventricular hypertrophy (PH-RVH) in sheep by left pulmonary artery (LPA) ligation combined with progressive main pulmonary artery (MPA) banding. This approach provides a controlled method to regulate RV afterload as tolerated by the animal in order to achieve PH-RVH, while reducing acute mortality. This animal model can facilitate evaluation of mechanical support devices for PH and RV failure.