JOINT RESURFACING USING ALLOGRAFT CHONDROCYTES AND SYNTHETIC BIODEGRADABLE POLYMER SCAFFOLDS
JOINT RESURFACING USING ALLOGRAFT CHONDROCYTES AND SYNTHETIC BIODEGRADABLE POLYMER SCAFFOLDS
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DOI：
10.1002/jbm.820280808
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发表时间：
1994-08-01
期刊：
影响因子：
--
通讯作者：
LANGER, R
中科院分区：
文献类型：
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作者：
FREED, LE;GRANDE, DA;LANGER, R
使用兔关节软骨细胞和合成的可生物降解的聚合物支架创建了可能用于修复受损关节的软骨植入物。将细胞连续传代，然后在纤维状聚乙醇酸（PGA）支架上体外培养。将细胞-PGA结构作为同种异体移植物植入体内，以修复成年兔膝关节中直径为3 mm的全层缺损，并在6个月内对软骨修复进行组织学评估。体外培养软骨细胞在PGA上增殖并再生软骨基质。在体内植入时，胶原蛋白和糖胺聚糖（GAG）分别占植入物干重（dw）的20 - 8%;其余为PGA和未指明组分。基于传代软骨细胞的植入物具有基于原代软骨细胞的植入物的1.7倍的GAG和2.6倍的胶原。在体内，PGA植入后，无论是否有培养的软骨细胞，都观察到软骨修复组织。细胞-PGA同种异体移植物的6个月修复在质量上优于PGA单独移植物，涉及：1）表面光滑度，2）软骨细胞的柱状排列，3）空间均匀的GAG分布，4）软骨下板的重建，以及5）修复组织与下层骨的结合。这些初步研究表明，使用细胞-聚合物同种异体移植物进行关节表面置换是可行的。(C)1994年，John Wiley and Sons，Inc.
Cartilage implants which could potentially be used to resurface damaged joints were created using rabbit articular chondrocytes and synthetic, biodegradable polymer scaffolds. Cells were serially passaged and then cultured in vitro on fibrous polyglycolic acid (PGA) scaffolds. Cell-PGA constructs were implanted in vivo as allografts to repair 3-mm diameter, full thickness defects in the knee joints of adult rabbits, and cartilage repair was assessed histologically over 6 months. In vitro, chondrocytes proliferated on PGA and regenerated cartilaginous matrix. Collagen and glycosaminoglycan (GAG) represented 20 to 8% of the implant dry weight (dw), respectively, at the time of in vivo implantation; the remainder was PGA and unspecified components. implants based on passaged chondrocytes had 1.7-times as much GAG and 2.6-times as much collagen as those based on primary chondrocytes. In vivo, cartilaginous repair tissue was observed after implantation of PGA both with and without cultured chondrocytes. Six month repair was qualitatively better for cell-PGA allografts than for PGA alone, with respect to: 1) surface smoothness, 2) columnar alignment of chondrocytes, 3) spatially uniform GAG distribution, 4) reconstitution of the subchondral plate, and 5) bonding of the repair tissue to the underlying bone. These pilot studies demonstrate that it is feasible to use cell-polymer allografts for joint resurfacing in vivo. (C) 1994 John Wiley and Sons, Inc.