Effects of interferon-gamma and tumor necrosis factor-alpha on the expression of an Ia antigen on a murine macrophage cell line.
Effects of interferon-gamma and tumor necrosis factor-alpha on the expression of an Ia antigen on a murine macrophage cell line.
复制标题
干扰素-γ 和肿瘤坏死因子-α 对小鼠巨噬细胞系 Ia 抗原表达的影响。
作者：
R. Chang;S. Lee
通过与rMuIFN-γ和/或rHuTNF-α孵育，可以诱导鼠骨髓单核细胞系WEHI-3的培养物表达II类MHC抗原I-A。用rMuIFN-γ（5.6 ng/ml）和rHuTNF-α（13 ng/ml）的组合孵育24小时诱导细胞群中的最高水平的表达（超过90%），其次是rMuIFN-γ（75%）和rHuTNF-α（33%）。I-A分子的重链亚基A α的mRNA水平的比较表明，该组合的刺激性分别是单独的rMuIFN-γ和rHuTNF-α的10倍和40倍。在检测的72小时孵育期间，每种细胞因子处理均诱导A α mRNA水平的时间依赖性增加，尽管组合继续将A α mRNA水平升高至高于任一细胞因子单独诱导的水平。本文报道的研究结果表明，单核因子rHuTNF-α可以诱导I-A抗原表达和A α mRNA。此外，相对于作为单独试剂的每种细胞因子的作用，rHuTNF-α和rMuIFN-γ的组合的刺激大于加和。
Cultures of the murine myelomonocytic cell line, WEHI-3, can be induced to express the Class II MHC antigen, I-A, by incubation with rMuIFN-gamma and/or rHuTNF-alpha. A 24-hr incubation with the combination of rMuIFN-gamma (5.6 ng/ml) and rHuTNF-alpha (13 ng/ml) induced the highest level of expression in the cell population (more than 90%), followed by rMuIFN-gamma (75%) and rHuTNF-alpha (33%). Comparison of the level of mRNA for the heavy chain subunit of the I-A molecule, A alpha, indicates that the combination is 10 and 40 times as stimulatory as rMuIFN-gamma and rHuTNF-alpha alone, respectively. Each cytokine treatment induced a time-dependent increase in the level of A alpha mRNA over the 72 hr of incubation examined, although the combination continued to elevate the level of A alpha mRNA above that induced by either cytokine alone. The findings reported here demonstrate that the monokine rHuTNF-alpha can induce I-A antigen expression and A alpha mRNA. Furthermore, stimulation by the combination of rHuTNF-alpha and rMuIFN-gamma is more than additive, relative to the effects of each cytokine as individual agents.