Formation and Stabilization of Gold Nanoparticles in Bovine Serum Albumin Solution
Formation and Stabilization of Gold Nanoparticles in Bovine Serum Albumin Solution
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DOI：
10.3390/molecules24183395
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发表时间：
2019-09-01
期刊：
影响因子：
4.6
通讯作者：
Ionita, Gabriela
中科院分区：
文献类型：
--
作者：
Matei, Iulia;Buta, Cristina Maria;Ionita, Gabriela
在pH为7的牛血清白蛋白（BSA）缓冲液中，研究了室温下金纳米颗粒（AuNPs）的形成和生长。采用紫外-可见光谱、圆二色光谱、拉曼光谱和电子顺磁共振（EPR）等技术对该过程进行了监测。用TEM显微镜和动态光散射（DLS）测量来证明纳米颗粒形成和生长过程中颗粒大小的变化。在pH为7时，在没有牛血清蛋白的情况下，没有观察到AuNPs的形成，这证明白蛋白参与了Au（III）还原的第一步。分别对BSA和AuNPs具有亲和力的两种自旋探针CAT16和DIS3的EPR光谱特征的变化，使我们能够监测颗粒的生长并证明BSA对AuNPs的保护作用。TEM显示，在BSA溶液中形成的AuNPs的大小随时间缓慢增加，形成不同形态的纳米颗粒。BSA的拉曼光谱表明白蛋白通过含硫氨基酸残基与AuNPs相互作用。本研究表明，白蛋白既是AuNPs的还原剂，又是保护冠。
The formation and growth of gold nanoparticles (AuNPs) were investigated in pH 7 buffer solution of bovine serum albumin (BSA) at room temperature. The processes were monitored by UV-Vis, circular dichroism, Raman and electron paramagnetic resonance (EPR) spectroscopies. TEM microscopy and dynamic light scattering (DLS) measurements were used to evidence changes in particle size during nanoparticle formation and growth. The formation of AuNPs at pH 7 in the absence of BSA was not observed, which proves that the albumin is involved in the first step of Au(III) reduction. Changes in the EPR spectral features of two spin probes, CAT16 and DIS3, with affinity for BSA and AuNPs, respectively, allowed us to monitor the particle growth and to demonstrate the protective role of BSA for AuNPs. The size of AuNPs formed in BSA solution increases slowly with time, resulting in nanoparticles of different morphologies, as revealed by TEM. Raman spectra of BSA indicate the interaction of albumin with AuNPs through sulfur-containing amino acid residues. This study shows that albumins act as both reducing agents and protective corona of AuNPs.