Hypocretin/orexin excites hypocretin neurons via a local glutamate neuron - A potential mechanism for orchestrating the hypothalamic arousal system
Hypocretin/orexin excites hypocretin neurons via a local glutamate neuron - A potential mechanism for orchestrating the hypothalamic arousal system
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DOI：
10.1016/s0896-6273(02)01132-7
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发表时间：
2002-12-19
期刊：
影响因子：
16.2
通讯作者：
van den Pol, AN
中科院分区：
文献类型：
--
作者：
Li, Y;Gao, XB;van den Pol, AN
释放下丘脑泌素/食欲素的神经元调节睡眠、唤醒和能量平衡；缺乏下丘脑分泌素会导致发作性睡病。在这里，我们提供了这些细胞的生理特征的数据，用转基因小鼠脑片中的GFP进行了鉴定。降克素-1和-2使降克汀神经元去极化15 mV，并诱发尖峰频率增加(较1-3赫兹基线增加366%)。其机制似乎是通过下丘脑素介导的局部谷氨酸能神经元的兴奋来调节下丘脑神经元的活动，部分是通过突触前促进谷氨酸的释放。这代表了一种可能的机制，用于协调弥漫下丘脑唤醒系统的输出。未检测到竹红菌素对其细胞膜性质的直接影响。去甲肾上腺素和5-羟色胺是其他唤醒系统的递质，它们降低了尖峰频率并诱发了外向电流，而乙酰胆碱和组胺几乎没有影响。
Neurons that release hypocretin/orexin modulate sleep, arousal, and energy homeostasis; the absence of hypocretin results in narcolepsy. Here we present data on the physiological characteristics of these cells, identified with GFP in transgenic mouse brain slices. Hypocretin-1 and -2 depolarized hypocretin neurons by 15mV and evoked an increase in spike frequency (+366% from a 1-3 Hz baseline). The mechanism for this appears to be hypocretin-mediated excitation of local glutamatergic neurons that regulate hypocretin neuron activity, in part by presynaptic facilitation of glutamate release. This represents a possible mechanism for orchestrating the output of the diffuse hypothalamic arousal system. No direct effect of hypocretin on membrane properties of hypocretin cells was detected. Norepinephrine and serotonin, transmitters of other arousal systems, decreased spike frequency and evoked outward currents, whereas acetylcholine and histamine had little effect.