Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells.
Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells.
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DOI：
10.1038/srep03652
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发表时间：
2014-01-13
影响因子：
4.6
通讯作者：
Chosa N
中科院分区：
文献类型：
--
作者：
Aomatsu E;Takahashi N;Sawada S;Okubo N;Hasegawa T;Taira M;Miura H;Ishisaki A;Chosa N
人类间充质干细胞（hMSCs）通过多种机制重塑或再生各种组织。在这里，我们确定了hMSC分泌蛋白SCRG 1及其受体BST 1作为自我更新，迁移和成骨分化的正调节因子。在hMSCs向成骨细胞分化的过程中，SCRG 1和BST 1基因表达下降。有趣的是，即使在高传代次数下，SCRG 1也能维持干细胞标志物（Oct-4和CD 271/LNGFR）的表达以及自我更新、迁移和成骨分化的潜力。因此，新的SCRG 1/BST 1轴通过调节其动力学和分化潜能来决定hMSC的命运。我们的研究结果提供了一个新的观点，体外扩增的hMSCs，保持天然干细胞的潜力，骨形成细胞治疗的方法。
Human mesenchymal stem cells (hMSCs) remodel or regenerate various tissues through several mechanisms. Here, we identified the hMSC-secreted protein SCRG1 and its receptor BST1 as a positive regulator of self-renewal, migration, and osteogenic differentiation. SCRG1 and BST1 gene expression decreased during osteogenic differentiation of hMSCs. Intriguingly, SCRG1 maintained stem cell marker expression (Oct-4 and CD271/LNGFR) and the potentials of self-renewal, migration, and osteogenic differentiation, even at high passage numbers. Thus, the novel SCRG1/BST1 axis determines the fate of hMSCs by regulating their kinetic and differentiation potentials. Our findings provide a new perspective on methods for ex vivo expansion of hMSCs that maintain native stem cell potentials for bone-forming cell therapy.
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影响因子：
5.9
作者：
Mabuchi, Yo;Morikawa, Satoru;Harada, Seiko;Niibe, Kunimichi;Suzuki, Sadafumi;Renault-Mihara, Francois;Houlihan, Diarmaid D.;Akazawa, Chihiro;Okano, Hideyuki;Matsuzaki, Yumi
通讯作者：
Matsuzaki, Yumi
影响因子：
--
作者：
Churchman, Sarah M.;Ponchel, Frederique;Jones, Elena A.
通讯作者：
Jones, Elena A.
影响因子：
6.5
作者：
Deschaseaux, F;Gindraux, F;Herve, P
通讯作者：
Herve, P
影响因子：
4
作者：
Halfon, Svetlana;Abramov, Natalie;Ginis, Irene
通讯作者：
Ginis, Irene
影响因子：
1.6
作者：
Hussain, AMM;Lee, HC;Chang, CF
通讯作者：
Chang, CF