Evaluation of genetic variation in the daylily (Hemerocallis spp.) using AFLP markers
Evaluation of genetic variation in the daylily (Hemerocallis spp.) using AFLP markers
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DOI：
10.1007/s001220051672
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发表时间：
2001-03
影响因子：
5.4
通讯作者：
Jeffrey Tomkins;Todd C. Wood;L. S. Barnes;A. Westman;R. A. Wing
中科院分区：
文献类型：
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作者：
Jeffrey Tomkins;Todd C. Wood;L. S. Barnes;A. Westman;R. A. Wing
黄花菜（Hemerocallisspp.）是商业上最重要的观赏植物之一。有趣的是，它也是过去60年中最重要的作物之一。自1947年美国萱草协会开始作为黄花菜品种的官方注册机构以来，已经处理了40000多个注册。为了确定集约化育种对品种发育的影响，并研究不同物种之间的关系，利用AFLP标记估计了黄花菜的遗传变异。19个主要基因型（种和早期品种）和100个现代品种，从不同的时间段使用152个明确的条带（平均79%的多态性率）来自3个AFLP引物组合进行了评估。总体而言，条目之间的成对相似性估计值在0.618和0.926之间（平均值=0.800）。当比较不同时期（1940-1998年）的栽培品种群时，与主要二倍体基因型相比，遗传相似性最初增加，从1940年到1980年保持不变，然后随着育种工作的加强和杂交者开始专注于通过秋水仙素转化获得的有限四倍体种质库而稳步增加。在现代黄花菜栽培品种（1991-1998），遗传相似性增加了约10%的主要基因型相比。这些数据也被用来评估黄花菜物种之间，除了一些小的例外，一般支持AFLP数据最近的分类分类。
The daylily (Hemerocallisspp.) is one of the most economically important ornamental plant species in commerce. Interestingly, it is also one of the most heavily bred crops during the past 60 years. Since the American Hemerocallis Society began acting as the official registry of daylily cultivars in 1947, more than 40 000 registrations have been processed. In order to determine the effects of intensive breeding on cultivar development, and to study relationships among different species, genetic variation in the daylily was estimated using AFLP markers. Nineteen primary genotypes (species and early cultivars) and 100 modern cultivars from different time periods were evaluated using 152 unambiguous bands (average 79% polymorphism rate) derived from three AFLP primer combinations. Overall, pairwise similarity estimates between entries ranged between 0.618 and 0.926 (average=0.800). When comparing cultivar groups from different time periods (1940–1998), genetic similarity was initially increased, compared to the primary diploid genotypes, remained constant from 1940 to 1980, and then steadily increased as breeding efforts intensified and hybridizers began focusing on a limited tetraploid germplasm pool derived by colchicine conversion. Among modern (1991–1998) daylily cultivars, genetic similarity has increased by approximately 10% compared to the primary genotypes. These data were also used to evaluate recent taxonomic classifications among daylily species which, with a few minor exceptions, were generally supported by the AFLP data.