Developmental expression of Pod 1 in Xenopus laevis.
Developmental expression of Pod 1 in Xenopus laevis.
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DOI：
10.1387/ijdb.051982ss
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
S. Simrick;K. Massé;E. Jones
中科院分区：
文献类型：
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作者：
S. Simrick;K. Massé;E. Jones
已显示碱性螺旋-环-螺旋转录因子Pod 1在许多发育中的小鼠器官（包括心脏、肺和肠道）的间充质中表达。在发育中小鼠的肾脏中，Pod 1在浓缩的后肾间充质、分化中的基质细胞和晚期基质细胞以及发育中的足细胞中高度表达。我们已经获得了一个EST（CF 270487），其中包含非洲爪蟾Pod 1序列。非洲爪蟾豆荚1序列的概念翻译显示约85%的相似性，其他脊椎动物同源物。RT-PCR表明，表达开始于第13阶段，并在发育中的原肾中与整个胚胎相比差异增加。42期肾脏解剖的RT-PCR显示，血管球中的表达高于肾小管或导管中的表达。在尾芽期的原位杂交分析显示，最前面的鳃弓和前肾球染色强烈。在第40阶段，染色持续存在于心脏的血管球和心外膜区域中。成人器官分析显示，在直肠和脾脏中表达最高，在十二指肠、心脏、肾脏、肺、胰腺、皮肤、肝脏和肌肉中表达显著。
The basic helix-loop-helix transcription factor, Pod 1, has been shown to be expressed in the mesenchyme of many developing mouse organs, including the heart, lungs and gut. In the kidneys of developing mice, Pod 1 is highly expressed in the condensing metanephric mesenchyme, differentiating and late stromal cells and in developing podocytes. We have obtained an EST (CF270487) which contains the Xenopus laevis Pod 1 sequence. Conceptual translation of the Xenopus laevis Pod 1 sequence shows approximately 85% similarity to other vertebrate homologues. RT-PCR indicates that expression is initiated at stage 13 and increases differentially in the developing pronephros compared to the whole embryo. RT-PCR of a kidney dissection at stage 42 shows higher expression in the glomus than in the tubule or duct. In situ hybridisation analysis at tail bud stages shows the anterior-most branchial arch and pronephric glomus are intensely stained. At stage 40, staining persists in the glomus and in the epicardium region of the heart. Adult organ analysis shows expression is highest in the rectum and the spleen, with significant expression in the duodenum, heart, kidney, lungs, pancreas, skin, liver and muscle.