Injectable extracellular matrix hydrogels contribute to native cell infiltration in a rat partial nephrectomy model
Injectable extracellular matrix hydrogels contribute to native cell infiltration in a rat partial nephrectomy model
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DOI：
10.1002/jbm.b.35144
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发表时间：
2022-08-08
影响因子：
3.4
通讯作者：
Kitagawa，Yuko
中科院分区：
文献类型：
--
作者：
Kushige，Hiroko;Amano，Yuki;Kitagawa，Yuko
脱细胞细胞外基质（dECM）水凝胶具有细胞相容性，并且目前正在研究作为促进天然细胞浸润和组织重建的材料在软组织中的应用。dECM水凝胶在具有复杂结构的器官或各种组织损伤模型中具有广泛的应用潜力。在这项研究中，我们通过将源自肾脏的dECM水凝胶注射到大鼠肾部分切除模型中来研究dECM水凝胶的实际应用。所制备的dECM水凝胶的粘度可调节，以允许保持在切除部位，并且在凝胶化后，具有与肾组织相似的弹性模量。此外，在体外观察了包埋在dECM水凝胶中的肾上皮细胞和血管内皮细胞的迁移，将dECM水凝胶注射到肾的部分切除部位4周后，在注射区域内观察到肾小管组成细胞和具有高增殖活性的天然细胞的浸润以及血管生成。这项研究首次表明，dECM水凝胶可以应用于肾脏，这是最复杂的结构器官之一，并且它们可以作为支架诱导血管生成和器官特异性肾小管组成细胞的浸润，为dECM水凝胶的进一步应用提供了基础见解。
Decellularized extracellular matrix (dECM) hydrogels have cytocompatibility, and are currently being investigated for application in soft tissues as a material that promotes native cell infiltration and tissue reconstruction. A dECM hydrogel has broad potential for application in organs with complex structures or various tissue injury models. In this study, we investigated the practical application of a dECM hydrogel by injecting a kidney‐derived dECM hydrogel into a rat partial nephrectomy model. The prepared dECM hydrogel was adjustable in viscosity to allow holding at the excision site, and after gelation, had an elastic modulus similar to that of kidney tissue. In addition, the migration of renal epithelial cells and vascular endothelial cells embedded in dECM hydrogels was observed in vitro.Four weeks after injection of the dECM hydrogel to the partial excision site of the kidneys, infiltration of renal tubular constituent cells and native cells with high proliferative activity, as well as angiogenesis, were observed inside the injected areas. This study is the first to show that dECM hydrogels can be applied to the kidney, one of the most complex structural organs and that they can function as a scaffold to induce angiogenesis and infiltration of organ‐specific renal tubular constituent cells, providing fundamental insights for further application of dECM hydrogels.